Agerarin, Identified from Ageratum Houstonianum, Stimulates Circadian CLOCK-mediated Aquaporin-3 Gene Expression in HaCaT Keratinocytes

Overview

Journal Sci Rep

Specialty Science

Date 2017 Sep 13

PMID 28894278

Citations 11

Authors

Soon Young Shin

Da Hyun Lee

Ha-Na Gil

Beom Soo Kim

Jeong-Sook Choe

Jung-Bong Kim

Young Han Lee

Yoongho Lim

Affiliations

Soon will be listed here.

Abstract

The juice of Ageratum houstonianum is used in folk medicine as an external wound healing aid for skin injuries. However, the active component of A. houstonianum and its mode of action in skin wound healing has not been investigated. This study was conducted to investigate the effect of A. houstonianum ethanolnolic extract (AHE) on the expression of aquaporin-3 (AQP3), an integral membrane protein for water and glycerol transport in keratinocytes, and to identify the structure of the A. houstonianum bioactive compound. Here, we show that AHE increased AQP3 gene expression at the transcriptional level through the p38 MAPK pathway in HaCaT cells. Furthermore, AHE ameliorated suppression of AQP3 expression caused by ultraviolet B (UVB) irradiation. Agerarin (6,7-dimethoxy-2,2-dimethyl-2H-chromene) was identified as the bioactive compound responsible for the up-regulation of AQP3 expression by enhancing the expression of the transcription factor circadian locomotor output cycles kaput (CLOCK). In conclusion, agerarin is a bioactive compound in AHE responsible for CLOCK-mediated AQP3 expression in keratinocytes.

Citing Articles

The Natural Janus Kinase Inhibitor Agerarin Downregulates Interleukin-4-Induced Expression in HaCaT Keratinocytes.

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Effect of 6,7-dimethoxy-2,2-dimethyl-2H-chromene (agerarin) on the recovery of filaggrin expression through targeting of Janus kinases in the inflammatory skin.

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Cosmeceutical Therapy: Engaging the Repercussions of UVR Photoaging on the Skin's Circadian Rhythm.

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Wound-Healing and Skin-Moisturizing Effects of Extract.

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