Reduction and Alkylation of Proteins in 2D Gel Electrophoresis: Before or After Isoelectric Focusing?
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References
1.
Herbert B, Galvani M, Hamdan M, Olivieri E, MacCarthy J, Pedersen S
. Reduction and alkylation of proteins in preparation of two-dimensional map analysis: why, when, and how?. Electrophoresis. 2001; 22(10):2046-57.
DOI: 10.1002/1522-2683(200106)22:10<2046::AID-ELPS2046>3.0.CO;2-C.
View
2.
Gorg A, Weiss W, Dunn M
. Current two-dimensional electrophoresis technology for proteomics. Proteomics. 2004; 4(12):3665-85.
DOI: 10.1002/pmic.200401031.
View
3.
Zhou S, Bailey M, Dunn M, Preedy V, Emery P
. A quantitative investigation into the losses of proteins at different stages of a two-dimensional gel electrophoresis procedure. Proteomics. 2005; 5(11):2739-47.
DOI: 10.1002/pmic.200401178.
View
4.
Tannu N, Hemby S
. Two-dimensional fluorescence difference gel electrophoresis for comparative proteomics profiling. Nat Protoc. 2007; 1(4):1732-42.
PMC: 2001252.
DOI: 10.1038/nprot.2006.256.
View
5.
Thelen J, Peck S
. Quantitative proteomics in plants: choices in abundance. Plant Cell. 2007; 19(11):3339-46.
PMC: 2174896.
DOI: 10.1105/tpc.107.053991.
View