» Articles » PMID: 28861411

Reduction and Alkylation of Proteins in 2D Gel Electrophoresis: Before or After Isoelectric Focusing?

Overview
Journal Front Chem
Specialty Chemistry
Date 2017 Sep 2
PMID 28861411
Citations 3
Authors
Affiliations
Soon will be listed here.
Citing Articles

Optimized Proteome Reduction for Integrative Top-Down Proteomics.

Woodland B, Necakov A, Coorssen J Proteomes. 2023; 11(1).

PMID: 36976889 PMC: 10059017. DOI: 10.3390/proteomes11010010.


Protein cysteine S-glycosylation: oxidative hydrolysis of protein S-glycosidic bonds in aqueous alkaline environments.

Buchowiecka A Amino Acids. 2022; 55(1):61-74.

PMID: 36460841 PMC: 9877059. DOI: 10.1007/s00726-022-03208-7.


Comparative proteomic analysis reveals the roots response to low root-zone temperature in Malus baccata.

Li L, Lu X, Ma H, Lyu D J Plant Res. 2018; 131(5):865-878.

PMID: 29855747 DOI: 10.1007/s10265-018-1045-6.

References
1.
Herbert B, Galvani M, Hamdan M, Olivieri E, MacCarthy J, Pedersen S . Reduction and alkylation of proteins in preparation of two-dimensional map analysis: why, when, and how?. Electrophoresis. 2001; 22(10):2046-57. DOI: 10.1002/1522-2683(200106)22:10<2046::AID-ELPS2046>3.0.CO;2-C. View

2.
Gorg A, Weiss W, Dunn M . Current two-dimensional electrophoresis technology for proteomics. Proteomics. 2004; 4(12):3665-85. DOI: 10.1002/pmic.200401031. View

3.
Zhou S, Bailey M, Dunn M, Preedy V, Emery P . A quantitative investigation into the losses of proteins at different stages of a two-dimensional gel electrophoresis procedure. Proteomics. 2005; 5(11):2739-47. DOI: 10.1002/pmic.200401178. View

4.
Tannu N, Hemby S . Two-dimensional fluorescence difference gel electrophoresis for comparative proteomics profiling. Nat Protoc. 2007; 1(4):1732-42. PMC: 2001252. DOI: 10.1038/nprot.2006.256. View

5.
Thelen J, Peck S . Quantitative proteomics in plants: choices in abundance. Plant Cell. 2007; 19(11):3339-46. PMC: 2174896. DOI: 10.1105/tpc.107.053991. View