Expression, Docking, and Molecular Dynamics of Endo--1,4-xylanase I Gene of in

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2017 Sep 1

PMID 28856159

Authors

Gammadde Hewa Ishan Maduka Wickramasinghe

Pilimathalawe Panditharathna Attanayake Mudiyanselage Samith Indika Rathnayake

Naduviladath Vishvanath Chandrasekharan

Mahindagoda Siril Samantha Weerasinghe

Ravindra Lakshman Chundananda Wijesundera

Wijepurage Sandhya Sulochana Wijesundera

Affiliations

Soon will be listed here.

Abstract

It is essential that major carbohydrate polymers in the lignocellulosic biomass are converted into fermentable sugars for the economical production of energy. Xylan, the major component of hemicelluloses, is the second most naturally abundant carbohydrate polymer comprising 20-40% of the total biomass. Endoxylanase (EXN) hydrolyzes xylan into mixtures of xylooligosaccharides. The objective of this study was to genetically modify , a pentose sugar fermenting yeast species, to hydrolyze xylan into xylooligosaccharides via cloning and heterologous extracellular expression of I gene from locally isolated species. was engineered to carry the I gene of using pGAPZ expression vector. The open reading frame encodes 191 amino acids and SDS-PAGE analysis revealed a 24 kDA recombinant protein. The EXNI activity expressed by recombinant clone under standard conditions using 1% beechwood xylan was 31.7 U/ml. Molecular docking and molecular dynamics simulations were performed to investigate EXNI-xylan interactions. Free EXNI and xylan bound EXNI exhibited similar stabilities and structural behavior in aqueous medium. Furthermore, this in silico work opens avenues for the development of newer generation EXN proteins that can perform better and have enhanced catalytic activity.

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