Genomes of Viral Isolates Derived from Different Mosquitos Species

Overview

Journal Virus Res

Specialty Microbiology

Date 2017 Sep 1

PMID 28855097

Citations 29

Authors

Mohammadreza Sadeghi

Vsevolod Popov

Hilda Guzman

Tung Gia Phan

Nikos Vasilakis

Robert Tesh

Eric Delwart

Affiliations

Soon will be listed here.

Abstract

Eleven viral isolates derived mostly in albopictus C6/36 cells from mosquito pools collected in Southeast Asia and the Americas between 1966 and 2014 contained particles with electron microscopy morphology typical of reoviruses. Metagenomics analysis yielded the near complete genomes of three novel reoviruses, Big Cypress orbivirus, Ninarumi virus, and High Island virus and a new tetravirus, Sarawak virus. Strains of previously characterized Sathuvarachi, Yunnan, Banna and Parry's Lagoon viruses (Reoviridae), Bontang virus (Mesoniviridae), and Culex theileri flavivirus (Flaviviridae) were also characterized. The availability of these mosquito virus genomes will facilitate their detection by metagenomics or PCR to better determine their geographic range, extent of host tropism, and possible association with arthropod or vertebrate disease.

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