Enhanced Arbovirus Surveillance with Deep Sequencing: Identification of Novel Rhabdoviruses and Bunyaviruses in Australian Mosquitoes

Overview

Journal Virology

Specialty Microbiology

Date 2013 Dec 10

PMID 24314645

Citations 72

Authors

Lark L Coffey

Brady L Page

Alexander L Greninger

Belinda L Herring

Richard C Russell

Stephen L Doggett

John Haniotis

Chunlin Wang

Xutao Deng

Eric L Delwart

Affiliations

Soon will be listed here.

Abstract

Viral metagenomics characterizes known and identifies unknown viruses based on sequence similarities to any previously sequenced viral genomes. A metagenomics approach was used to identify virus sequences in Australian mosquitoes causing cytopathic effects in inoculated mammalian cell cultures. Sequence comparisons revealed strains of Liao Ning virus (Reovirus, Seadornavirus), previously detected only in China, livestock-infecting Stretch Lagoon virus (Reovirus, Orbivirus), two novel dimarhabdoviruses, named Beaumont and North Creek viruses, and two novel orthobunyaviruses, named Murrumbidgee and Salt Ash viruses. The novel virus proteomes diverged by ≥ 50% relative to their closest previously genetically characterized viral relatives. Deep sequencing also generated genomes of Warrego and Wallal viruses, orbiviruses linked to kangaroo blindness, whose genomes had not been fully characterized. This study highlights viral metagenomics in concert with traditional arbovirus surveillance to characterize known and new arboviruses in field-collected mosquitoes. Follow-up epidemiological studies are required to determine whether the novel viruses infect humans.

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References

Knope K, Whelan P, Smith D, Johansen C, Moran R, Doggett S . Arboviral diseases and malaria in Australia, 2010-11: annual report of the National Arbovirus and Malaria Advisory Committee. Commun Dis Intell Q Rep. 2013; 37(1):E1-20. View

Foley D, Wilkerson R, Cooper R, Volovsek M, Bryan J . A molecular phylogeny of Anopheles annulipes (Diptera: Culicidae) sensu lato: the most species-rich anopheline complex. Mol Phylogenet Evol. 2006; 43(1):283-97. DOI: 10.1016/j.ympev.2006.10.008. View

Reddacliff L, Kirkland P, Philbey A, Davis R, Vogelnest L, Hulst F . Experimental reproduction of viral chorioretinitis in kangaroos. Aust Vet J. 1999; 77(8):522-8. DOI: 10.1111/j.1751-0813.1999.tb12125.x. View

Jansen C, Prow N, Webb C, Hall R, Pyke A, Harrower B . Arboviruses isolated from mosquitoes collected from urban and peri-urban areas of eastern Australia. J Am Mosq Control Assoc. 2009; 25(3):272-8. DOI: 10.2987/09-5908.1. View

STANDFAST H, DYCE A, St George T, Muller M, Doherty R, CARLEY J . Isolation of arboviruses from insects collected at Beatrice Hill, Northern Territory of Australia, 1974-1976. Aust J Biol Sci. 1984; 37(5-6):351-66. DOI: 10.1071/bi9840351. View

Quan P, Junglen S, Tashmukhamedova A, Conlan S, Hutchison S, Kurth A . Moussa virus: a new member of the Rhabdoviridae family isolated from Culex decens mosquitoes in Côte d'Ivoire. Virus Res. 2009; 147(1):17-24. PMC: 2791192. DOI: 10.1016/j.virusres.2009.09.013. View

Grard G, Fair J, Lee D, Slikas E, Steffen I, Muyembe J . A novel rhabdovirus associated with acute hemorrhagic fever in central Africa. PLoS Pathog. 2012; 8(9):e1002924. PMC: 3460624. DOI: 10.1371/journal.ppat.1002924. View

Ruby J, Bellare P, DeRisi J . PRICE: software for the targeted assembly of components of (Meta) genomic sequence data. G3 (Bethesda). 2013; 3(5):865-80. PMC: 3656733. DOI: 10.1534/g3.113.005967. View

Monath T, Cropp C, Frazier C, Murphy F, Whitfield S . Viruses isolated from reptiles: identification of three new members of the family Rhabdoviridae. Arch Virol. 1979; 60(1):1-12. DOI: 10.1007/BF01318092. View

10.

Walter C, Barr J . Recent advances in the molecular and cellular biology of bunyaviruses. J Gen Virol. 2011; 92(Pt 11):2467-2484. DOI: 10.1099/vir.0.035105-0. View

11.

Hall-Mendelin S, Allcock R, Kresoje N, van den Hurk A, Warrilow D . Detection of arboviruses and other micro-organisms in experimentally infected mosquitoes using massively parallel sequencing. PLoS One. 2013; 8(2):e58026. PMC: 3584025. DOI: 10.1371/journal.pone.0058026. View

12.

Powers A, Logue C . Changing patterns of chikungunya virus: re-emergence of a zoonotic arbovirus. J Gen Virol. 2007; 88(Pt 9):2363-2377. DOI: 10.1099/vir.0.82858-0. View

13.

Savji N, Palacios G, da Rosa A, Hutchison S, Celone C, Hui J . Genomic and phylogenetic characterization of Leanyer virus, a novel orthobunyavirus isolated in northern Australia. J Gen Virol. 2011; 92(Pt 7):1676-1687. PMC: 3167897. DOI: 10.1099/vir.0.028308-0. View

14.

Nabeshima T, Nga P, Guillermo P, Parquet M, Yu F, Thuy N . Isolation and molecular characterization of Banna virus from mosquitoes, Vietnam. Emerg Infect Dis. 2008; 14(8):1276-9. PMC: 2600385. DOI: 10.3201/eid1408.080100. View

15.

Tao S, Chen B . Studies of coltivirus in China. Chin Med J (Engl). 2005; 118(7):581-6. View

16.

Blacksell S, Lunt R, White J . Rapid identification of Australian bunyavirus isolates belonging to the Simbu serogroup using indirect ELISA formats. J Virol Methods. 1997; 66(1):123-33. DOI: 10.1016/s0166-0934(97)00046-3. View

17.

Swei A, Russell B, Naccache S, Kabre B, Veeraraghavan N, Pilgard M . The genome sequence of Lone Star virus, a highly divergent bunyavirus found in the Amblyomma americanum tick. PLoS One. 2013; 8(4):e62083. PMC: 3639253. DOI: 10.1371/journal.pone.0062083. View

18.

Ng T, Willner D, Lim Y, Schmieder R, Chau B, Nilsson C . Broad surveys of DNA viral diversity obtained through viral metagenomics of mosquitoes. PLoS One. 2011; 6(6):e20579. PMC: 3108952. DOI: 10.1371/journal.pone.0020579. View

19.

Azuolas J, Wishart E, Bibby S, Ainsworth C . Isolation of Ross River virus from mosquitoes and from horses with signs of musculo-skeletal disease. Aust Vet J. 2004; 81(6):344-7. DOI: 10.1111/j.1751-0813.2003.tb11511.x. View

20.

Attoui H, Mohd Jaafar F, Belhouchet M, Tao S, Chen B, Liang G . Liao ning virus, a new Chinese seadornavirus that replicates in transformed and embryonic mammalian cells. J Gen Virol. 2005; 87(Pt 1):199-208. DOI: 10.1099/vir.0.81294-0. View