Seq-Well: Portable, Low-cost RNA Sequencing of Single Cells at High Throughput

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2017 Feb 14

PMID 28192419

Citations 440

Authors

Todd M Gierahn

Marc H Wadsworth 2nd

Travis K Hughes

Bryan D Bryson

Andrew Butler

Rahul Satija

Sarah Fortune

J Christopher Love

Alex K Shalek

Affiliations

Soon will be listed here.

Abstract

Single-cell RNA-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA-seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We use Seq-Well to profile thousands of primary human macrophages exposed to Mycobacterium tuberculosis.

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