Amino Acid Substitution in the Major Multidrug Efflux Transporter Protein AcrB Contributes to Low Susceptibility to Azithromycin in Haemophilus Influenzae

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2017 Aug 30

PMID 28848006

Citations 7

Authors

Shoji Seyama

Takeaki Wajima

Hidemasa Nakaminami

Norihisa Noguchi

Affiliations

Soon will be listed here.

Abstract

Clarithromycin-resistant strains with a nonsense mutation in generally exhibited susceptibility to azithromycin, although one strain was found to be nonsusceptible; we aimed to clarify the differences. This strain had an amino acid substitution, Arg327Ser, in AcrB. Introduction of this substitution into Rd caused an increase in the MIC of azithromycin, suggesting that this substitution contributed to nonsusceptibility. These findings indicate that azithromycin-nonsusceptible isolates could occur through stepwise mutation in the region.

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