Molecular Cloning of CDNA Coding for Rat Proliferating Cell Nuclear Antigen (PCNA)/cyclin

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1987 Mar 1

PMID 2884104

Citations 48

Authors

K Matsumoto

T Moriuchi

T Koji

P K Nakane

Affiliations

Soon will be listed here.

Abstract

The 'proliferating cell nuclear antigen' (PCNA), also known as cyclin, appears at the G1/S boundary in the cell cycle. Because of its possible relationship with cell proliferation, PCNA/cyclin has been receiving attention. PCNA/cyclin is a non-histone acidic nuclear protein with an apparent mol. wt of 33000-36000. The amino acid composition and the sequence of the first 25 amino acids of rabbit PCNA/cyclin are known. Using an oligonucleotide probe corresponding to the sequence of the first five amino acids, a cDNA clone for PCNA/cyclin was isolated from rat thymocyte cDNA library. The cDNA (1195 bases) contains an open reading frame of 813 nucleotides coding for 261 amino acids. The 3'-non-coding region is 312 nucleotides long and contains three putative polyadenylation signals. The mol. wt of rat PCNA/cyclin was calculated to be 28 748. The deduced amino acid sequence and composition of rat PCNA/cyclin are in excellent agreement with the published data. Using the cDNA probe, two species of mRNA (1.1 and 0.98 kb) were detected in rat thymocyte RNA. Southern blot analysis of total human genomic DNA suggests that there is a single gene coding for PCNA/cyclin. The deduced amino acid sequence of rat PCNA/cyclin has a similarity with that of herpes simplex virus type-1 DNA binding protein.

Citing Articles

Prospective Molecular Targets for Natural Killer Cell Immunotherapy against Glioblastoma Multiforme.

Cooksey L, Friesen D, Mangan E, Mathew P Cells. 2024; 13(18.

PMID: 39329751 PMC: 11429815. DOI: 10.3390/cells13181567.

Bioinformatics analysis of the prognostic biomarkers and predictive accuracy of differentially expressed genes in high-risk multiple myeloma based on Gene Expression Omnibus database mining.

Du C, Guo D, Zhang Y, Gao C, Bai J Ann Transl Med. 2023; 10(24):1325.

PMID: 36660705 PMC: 9843371. DOI: 10.21037/atm-22-2656.

Comparative Proteomic Analysis of Drug Trichosanthin Addition to BeWo Cell Line.

Hu Y, Yao J, Wang Z, Liang H, Li C, Zhou X Molecules. 2022; 27(5).

PMID: 35268705 PMC: 8911981. DOI: 10.3390/molecules27051603.

Investigation of the effect of metoclopramide on proliferation signal molecules in breast tissue.

Umur N, Ildan Calim S, Yazici G, Gurgen S Int J Exp Pathol. 2022; 103(3):83-89.

PMID: 35243705 PMC: 9107609. DOI: 10.1111/iep.12433.

Molecular modeling and expression of the Litopenaeus vannamei proliferating cell nuclear antigen (PCNA) after white spot syndrome virus shrimp infection.

De-la-Re-Vega E, Muhlia-Almazan A, Arvizu-Flores A, Islas-Osuna M, Yepiz-Plascencia G, Brieba L Results Immunol. 2013; 1(1):24-30.

PMID: 24371549 PMC: 3787728. DOI: 10.1016/j.rinim.2011.07.001.

References

McLachlan A . Tests for comparing related amino-acid sequences. Cytochrome c and cytochrome c 551 . J Mol Biol. 1971; 61(2):409-24. DOI: 10.1016/0022-2836(71)90390-1. View

Honess R, Roizman B . Proteins specified by herpes simplex virus. XI. Identification and relative molar rates of synthesis of structural and nonstructural herpes virus polypeptides in the infected cell. J Virol. 1973; 12(6):1347-65. PMC: 356776. DOI: 10.1128/JVI.12.6.1347-1365.1973. View

Honess R, Roizman B . Regulation of herpesvirus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins. J Virol. 1974; 14(1):8-19. PMC: 355471. DOI: 10.1128/JVI.14.1.8-19.1974. View

Miyachi K, Fritzler M, Tan E . Autoantibody to a nuclear antigen in proliferating cells. J Immunol. 1978; 121(6):2228-34. View

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

Perricaudet M, Akusjarvi G, Virtanen A, Pettersson U . Structure of two spliced mRNAs from the transforming region of human subgroup C adenoviruses. Nature. 1979; 281(5733):694-6. DOI: 10.1038/281694a0. View

Conley A, Knipe D, Jones P, Roizman B . Molecular genetics of herpes simplex virus. VII. Characterization of a temperature-sensitive mutant produced by in vitro mutagenesis and defective in DNA synthesis and accumulation of gamma polypeptides. J Virol. 1981; 37(1):191-206. PMC: 170996. DOI: 10.1128/JVI.37.1.191-206.1981. View

Smart J, Lewis J, Mathews M, Harter M, Anderson C . Adenovirus type 2 early proteins: assignment of the early region 1A proteins synthesized in vivo and in vitro to specific mRNAs. Virology. 1981; 112(2):703-13. DOI: 10.1016/0042-6822(81)90315-9. View

Powell K, Littler E, Purifoy D . Nonstructural proteins of herpes simplex virus. II. Major virus-specific DNa-binding protein. J Virol. 1981; 39(3):894-902. PMC: 171323. DOI: 10.1128/JVI.39.3.894-902.1981. View

10.

Kruijer W, van Schaik F, Sussenbach J . Structure and organization of the gene coding for the DNA binding protein of adenovirus type 5. Nucleic Acids Res. 1981; 9(18):4439-57. PMC: 327449. DOI: 10.1093/nar/9.18.4439. View

11.

Bravo R, Fey S, Bellatin J, Larsen P, Arevalo J, Celis J . Identification of a nuclear and of a cytoplasmic polypeptide whose relative proportions are sensitive to changes in the rate of cell proliferation. Exp Cell Res. 1981; 136(2):311-9. DOI: 10.1016/0014-4827(81)90009-4. View

12.

Takasaki Y, Deng J, Tan E . A nuclear antigen associated with cell proliferation and blast transformation. J Exp Med. 1981; 154(6):1899-909. PMC: 2186555. DOI: 10.1084/jem.154.6.1899. View

13.

Gingeras T, Sciaky D, Gelinas R, Yen C, Kelly M, Bullock P . Nucleotide sequences from the adenovirus-2 genome. J Biol Chem. 1982; 257(22):13475-91. View

14.

Moriuchi T, Chang H, Denome R, Silver J . Thy-1 cDNA sequence suggests a novel regulatory mechanism. Nature. 1983; 301(5895):80-2. DOI: 10.1038/301080a0. View

15.

Watt R, Stanton L, Marcu K, Gallo R, Croce C, Rovera G . Nucleotide sequence of cloned cDNA of human c-myc oncogene. Nature. 1983; 303(5919):725-8. DOI: 10.1038/303725a0. View

16.

van Straaten F, Muller R, Curran T, Van Beveren C, Verma I . Complete nucleotide sequence of a human c-onc gene: deduced amino acid sequence of the human c-fos protein. Proc Natl Acad Sci U S A. 1983; 80(11):3183-7. PMC: 394004. DOI: 10.1073/pnas.80.11.3183. View

17.

Ohlendorf D, Matthews B . Structural studies of protein-nucleic acid interactions. Annu Rev Biophys Bioeng. 1983; 12:259-84. DOI: 10.1146/annurev.bb.12.060183.001355. View

18.

Kozak M . Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res. 1984; 12(2):857-72. PMC: 318541. DOI: 10.1093/nar/12.2.857. View

19.

Quinlan M, Chen L, Knipe D . The intranuclear location of a herpes simplex virus DNA-binding protein is determined by the status of viral DNA replication. Cell. 1984; 36(4):857-68. DOI: 10.1016/0092-8674(84)90035-7. View

20.

Takasaki Y, Fishwild D, Tan E . Characterization of proliferating cell nuclear antigen recognized by autoantibodies in lupus sera. J Exp Med. 1984; 159(4):981-92. PMC: 2187268. DOI: 10.1084/jem.159.4.981. View