Rapid Detection of Serratia Fonticola by TaqMan Quantitative Real-Time PCR Using Primers Targeting the GyrB Gene

Overview

Journal Curr Microbiol

Specialty Microbiology

Date 2017 Aug 20

PMID 28821942

Citations 8

Authors

Jing Hua Ruan

Wu Jun Wang

Ti Yin Zhang

Quan Yang Bai

Teng Zheng

Zhi Deng Zhang

Li Yun Wu

Yi Fan Huang

Dao Jin Yu

Affiliations

Soon will be listed here.

Abstract

A gyrB gene is present in the majority of bacterial species, and encodes the ATPase domain of DNA gyraseB-subunit protein, which is essential for transcription and replication of bacteria. The gyrB gene exhibits higher nucleotide sequence variability than the 16S rDNA gene and thus could be more reliable in differentiating Serratia fonticola. A species-specific primer pair and probe were designed for quantitative real-time PCR detection of S. fonticola using gyrB as the target gene. Nine members of the Serratia family (representing nine Serratia species) were chosen to verify the specificity of the primers. Additionally, two species each of Salmonella and Klebsiella, and five other species belonging to five other genera of Enterobacteriaceae, were tested for primer cross-reaction. All the tested strains gave negative results. The limit of detection for S. fonticola using the gyrB gene was 100 copies per PCR reaction. This TaqMan PCR assay provided a specific, rapid, and sensitive method to detect S. fonticola based on its gyrB gene.

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