DNA Gyrase: an Enzyme That Introduces Superhelical Turns into DNA

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1976 Nov 1

PMID 186775

Citations 483

Authors

M Gellert

K Mizuuchi

M H ODea

H A NASH

Affiliations

Soon will be listed here.

Abstract

Relaxed closed-circular DNA is converted to negatively supercoiled DNA by DNA gyrase. This enzyme has been purified from Escherichia coli cells. The reaction requires ATP and Mg++ and is stimulated by spermidine. The enzyme acts equally well on relaxed closed-circular colicin E1, phage lambda, and simian virus 40 DNA. The final superhelix density of the DNA can be considerably greater than that found in intracellularly supercoiled DNA.

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