CD177 Modulates Human Neutrophil Migration Through Activation-mediated Integrin and Chemoreceptor Regulation

Overview

Journal Blood

Publisher Elsevier

Specialty Hematology

Date 2017 Aug 16

PMID 28807980

Citations 77

Authors

Ming Bai

Ricardo Grieshaber-Bouyer

Junxia Wang

Angela B Schmider

Zachary S Wilson

Liling Zeng

Olha Halyabar

Matthew D Godin

Hung N Nguyen

Anais Levescot

Pierre Cunin

Craig T Lefort

Roy J Soberman

Peter A Nigrovic

Affiliations

Soon will be listed here.

Abstract

CD177 is a glycosylphosphatidylinositol (GPI)-anchored protein expressed by a variable proportion of human neutrophils that mediates surface expression of the antineutrophil cytoplasmic antibody antigen proteinase 3. CD177 associates with β2 integrins and recognizes platelet endothelial cell adhesion molecule 1 (PECAM-1), suggesting a role in neutrophil migration. However, CD177 neutrophils exhibit no clear migratory advantage in vivo, despite interruption of in vitro transendothelial migration by CD177 ligation. We sought to understand this paradox. Using a PECAM-1-independent transwell system, we found that CD177 and CD177 neutrophils migrated comparably. CD177 ligation selectively impaired migration of CD177 neutrophils, an effect mediated through immobilization and cellular spreading on the transwell membrane. Correspondingly, CD177 ligation enhanced its interaction with β2 integrins, as revealed by fluorescence lifetime imaging microscopy, leading to integrin-mediated phosphorylation of Src and extracellular signal-regulated kinase (ERK). CD177-driven cell activation enhanced surface β2 integrin expression and affinity, impaired internalization of integrin attachments, and resulted in ERK-mediated attenuation of chemokine signaling. We conclude that CD177 signals in a β2 integrin-dependent manner to orchestrate a set of activation-mediated mechanisms that impair human neutrophil migration.

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