Role of Transcription Regulatory Sequence in Regulation of Gene Expression and Replication of Porcine Reproductive and Respiratory Syndrome Virus

Overview

Journal Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2017 Aug 12

PMID 28797297

Citations 5

Authors

Chengbao Wang

Han Meng

Yujin Gao

Hui Gao

Kangkang Guo

Fernando Almazan

Isabel Sola

Luis Enjuanes

Yanming Zhang

Levon Abrahamyan

Affiliations

Soon will be listed here.

Abstract

In order to gain insight into the role of the transcription regulatory sequences (TRSs) in the regulation of gene expression and replication of porcine reproductive and respiratory syndrome virus (PRRSV), the enhanced green fluorescent protein (EGFP) gene, under the control of the different structural gene TRSs, was inserted between the N gene and 3'-UTR of the PRRSV genome and EGFP expression was analyzed for each TRS. TRSs of all the studied structural genes of PRRSV positively modulated EGFP expression at different levels. Among the TRSs analyzed, those of GP2, GP5, M, and N genes highly enhanced EGFP expression without altering replication of PRRSV. These data indicated that structural gene TRSs could be an extremely useful tool for foreign gene expression using PRRSV as a vector.

Citing Articles

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Erratum to: Role of transcription regulatory sequence in regulation of gene expression and replication of porcine reproductive and respiratory syndrome virus.

Wang C, Meng H, Gao Y, Gao H, Guo K, Almazan F Vet Res. 2017; 48(1):54.

PMID: 28931424 PMC: 5607586. DOI: 10.1186/s13567-017-0464-z.

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