Fractioning of Proanthocyanidins of Uncaria Tomentosa. Composition and Structure-Bioactivity Relationship

Overview

Journal Antioxidants (Basel)

Date 2017 Aug 10

PMID 28788071

Citations 9

Authors

Mirtha Navarro

William Zamora

Silvia Quesada

Gabriela Azofeifa

Diego Alvarado

Maria Monagas

Affiliations

Soon will be listed here.

Abstract

In a previous study, the detailed low-molecular weight polyphenolic profile of the different plant parts (leaves, stem, bark and wood) of was reported, the leaves being the plant part with the highest phenolic content and presenting the most heterogenous proanthocyanidin composition. Further, cytotoxicity of leaves extracts in two cancer cell lines was also found to be higher than in the remaining parts of the plant. In the present study, fractioning of leaves polyphenolic extracts was performed using Diaion HP-20 resin and a detailed characterization and quantification of fractions ( = 5) was achieved using advanced analytical techniques such as Ultra-Performance Liquid Chromatography coupled with Electrospray Ionization and Triple Quadrupole (TQD) Tandem Mass Spectrometry (UPLC/TQ-ESI-MS) and C-NMR. Oxygen Radical Absorbance Capacity (ORAC) and cytotoxicity on gastric adenocarcinoma AGS and colon adenocarcinoma SW20 cell lines were also determined in the different fractions. Results showed selective distribution of 32 non-flavonoid and flavonoid phenolics among the different fractions. ORAC varied between 3.2 and 11.8 μmol TE/mg in the different fractions, whereas IC of cytotoxicity on gastric adenocarcinoma AGS and colon adenocarcinoma SW20 cell lines best values were between 71.4 and 75.6 µg/mL. Fractions rich in proanthocyanidins also showed the highest bioactivity. In fact, significant positive correlation was found between total proanthocyanidins (TP) quantified by UPLC-DAD and ORAC (² = 0.970), whereas significant negative correlation was found between TP and cytotoxicity towards AGS (² = 0.820) and SW620 (² = 0.843) adenocarcinoma cell lines. Among proanthocyanidins, propelargonidin dimers were of particular interest, showing significant correlation with cytotoxic selectivity on both gastric AGS (² = 0.848) and colon SW620 (² = 0.883) adenocarcinoma cell lines. These results show further evidence of the bioactivity of proanthocyanidin extracts and their potential health effects.

Citing Articles

Hybrid Nanoparticles of Proanthocyanidins from Leaves: QTOF-ESI MS Characterization, Antioxidant Activity and Immune Cellular Response.

Araya-Sibaja A, Wilhelm-Romero K, Vargas-Huertas F, Quiros-Fallas M, Alvarado-Corella D, Mora-Roman J Plants (Basel). 2022; 11(13).

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Polyphenolic QTOF-ESI MS Characterization and the Antioxidant and Cytotoxic Activities of Commercial Cultivars from Costa Rica.

Navarro-Hoyos M, Arnaez-Serrano E, Quesada-Mora S, Azofeifa-Cordero G, Wilhelm-Romero K, Quiros-Fallas M Molecules. 2021; 26(21).

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Investigating Potential Inhibitory Effect of (Cat's Claw) against the Main Protease 3CL of SARS-CoV-2 by Molecular Modeling.

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LC-MS and Spectrophotometric Approaches for Evaluation of Bioactive Compounds from Peru Cocoa By-Products for Commercial Applications.

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Polyphenolic Characterization, Antioxidant, and Cytotoxic Activities of Cultivars from Costa Rica.

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