Location of the 100 Kd-50 Kd Accessory Proteins in Clathrin Coats

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1986 Sep 1

PMID 2877872

Citations 51

Authors

G P Vigers

R A Crowther

B M Pearse

Affiliations

Soon will be listed here.

Abstract

We present a three-dimensional map of the clathrin coat of coated vesicles, generated from tilt series of electron micrographs of unstained specimens embedded in vitreous ice. We have examined native placental coated vesicles and coats reassembled from their purified constituents, namely clathrin triskelions and accessory proteins of approximate mol. wts 100 kd and 50 kd. Our results show that the accessory proteins contribute a further shell of density within the double shell of the clathrin cage, extending from the terminal domains of the clathrin to the membrane of the vesicle. The thickness of the complete coat is approximately 22 nm.

Citing Articles

Passive diffusion accounts for the majority of intracellular nanovesicle transport.

Sittewelle M, Royle S Life Sci Alliance. 2023; 7(1).

PMID: 37857498 PMC: 10587482. DOI: 10.26508/lsa.202302406.

The amount of Nck rather than N-WASP correlates with the rate of actin-based motility of Vaccinia virus.

Basant A, Way M Microbiol Spectr. 2023; 11(6):e0152923.

PMID: 37855608 PMC: 10883800. DOI: 10.1128/spectrum.01529-23.

Understanding Intracellular Biology to Improve mRNA Delivery by Lipid Nanoparticles.

Hunter M, Cui L, Porebski B, Pereira S, Sonzini S, Odunze U Small Methods. 2023; 7(9):e2201695.

PMID: 37317010 PMC: 7615154. DOI: 10.1002/smtd.202201695.

A multiscale model of the regulation of aquaporin 2 recycling.

Leberecht C, Schroeder M, Labudde D NPJ Syst Biol Appl. 2022; 8(1):16.

PMID: 35534498 PMC: 9085758. DOI: 10.1038/s41540-022-00223-y.

Thermoresponsive Chitosan-Grafted-Poly(-vinylcaprolactam) Microgels via Ionotropic Gelation for Oncological Applications.

Marsili L, Dal Bo M, Berti F, Toffoli G Pharmaceutics. 2021; 13(10).

PMID: 34683947 PMC: 8539247. DOI: 10.3390/pharmaceutics13101654.

References

Huang C . Studies on phosphatidylcholine vesicles. Formation and physical characteristics. Biochemistry. 1969; 8(1):344-52. DOI: 10.1021/bi00829a048. View

Goldstein J, Brown M, Anderson R, Russell D, Schneider W . Receptor-mediated endocytosis: concepts emerging from the LDL receptor system. Annu Rev Cell Biol. 1985; 1:1-39. DOI: 10.1146/annurev.cb.01.110185.000245. View

Fenner C, Traut R, Mason D, Wikman-Coffelt J . Quantification of Coomassie Blue stained proteins in polyacrylamide gels based on analyses of eluted dye. Anal Biochem. 1975; 63(2):595-602. DOI: 10.1016/0003-2697(75)90386-3. View

Crowther R, Finch J, Pearse B . On the structure of coated vesicles. J Mol Biol. 1976; 103(4):785-98. DOI: 10.1016/0022-2836(76)90209-6. View

Goldstein J, Anderson R, Brown M . Coated pits, coated vesicles, and receptor-mediated endocytosis. Nature. 1979; 279(5715):679-85. DOI: 10.1038/279679a0. View

BRETSCHER M, Thomson J, Pearse B . Coated pits act as molecular filters. Proc Natl Acad Sci U S A. 1980; 77(7):4156-9. PMC: 349789. DOI: 10.1073/pnas.77.7.4156. View

Ungewickell E, Branton D . Assembly units of clathrin coats. Nature. 1981; 289(5796):420-2. DOI: 10.1038/289420a0. View

Pearse B, BRETSCHER M . Membrane recycling by coated vesicles. Annu Rev Biochem. 1981; 50:85-101. DOI: 10.1146/annurev.bi.50.070181.000505. View

Schmid S, Matsumoto A, Rothman J . A domain of clathrin that forms coats. Proc Natl Acad Sci U S A. 1982; 79(1):91-5. PMC: 345667. DOI: 10.1073/pnas.79.1.91. View

10.

UNANUE E, Ungewickell E, Branton D . The binding of clathrin triskelions to membranes from coated vesicles. Cell. 1981; 26(3 Pt 1):439-46. DOI: 10.1016/0092-8674(81)90213-0. View

11.

Crowther R, Pearse B . Assembly and packing of clathrin into coats. J Cell Biol. 1981; 91(3 Pt 1):790-7. PMC: 2112829. DOI: 10.1083/jcb.91.3.790. View

12.

Pearse B . Coated vesicles from human placenta carry ferritin, transferrin, and immunoglobulin G. Proc Natl Acad Sci U S A. 1982; 79(2):451-5. PMC: 345761. DOI: 10.1073/pnas.79.2.451. View

13.

Ungewickell E, UNANUE E, Branton D . Functional and structural studies on clathrin triskelions and baskets. Cold Spring Harb Symp Quant Biol. 1982; 46 Pt 2:723-31. DOI: 10.1101/sqb.1982.046.01.069. View

14.

Zaremba S, Keen J . Assembly polypeptides from coated vesicles mediate reassembly of unique clathrin coats. J Cell Biol. 1983; 97(5 Pt 1):1339-47. PMC: 2112702. DOI: 10.1083/jcb.97.5.1339. View

15.

Lepault J, Pitt T . Projected structure of unstained, frozen-hydrated T-layer of Bacillus brevis. EMBO J. 1984; 3(1):101-5. PMC: 557304. DOI: 10.1002/j.1460-2075.1984.tb01768.x. View

16.

Hanspal M, Luna E, Branton D . The association of clathrin fragments with coated vesicle membranes. J Biol Chem. 1984; 259(17):11075-82. View

17.

Pearse B, Robinson M . Purification and properties of 100-kd proteins from coated vesicles and their reconstitution with clathrin. EMBO J. 1984; 3(9):1951-7. PMC: 557627. DOI: 10.1002/j.1460-2075.1984.tb02075.x. View

18.

Kirchhausen T, Harrison S . Structural domains of clathrin heavy chains. J Cell Biol. 1984; 99(5):1725-34. PMC: 2113343. DOI: 10.1083/jcb.99.5.1725. View

19.

Stewart M, Vigers G . Electron microscopy of frozen-hydrated biological material. Nature. 1986; 319(6055):631-6. DOI: 10.1038/319631a0. View

20.

Vigers G, Crowther R, Pearse B . Three-dimensional structure of clathrin cages in ice. EMBO J. 1986; 5(3):529-34. PMC: 1166794. DOI: 10.1002/j.1460-2075.1986.tb04242.x. View