Design Mechanism and Property of the Novel Fluorescent Probes for the Identification of Microthrix Parvicella In Situ

Overview

Journal Materials (Basel)

Publisher MDPI

Date 2017 Aug 5

PMID 28773166

Citations 4

Authors

Xiumei Jiao

Xuening Fei

Songya Li

Dayong Lin

Huaji Ma

Baolian Zhang

Affiliations

Soon will be listed here.

Abstract

In this study, two novel fluorescent probes, probe A and probe B were designed, synthesized and characterized, based on () preferring to utilize long-chain fatty acid (LCFA), for the labeling of in activated sludge. The molecular structure of probe A and probe B include long-chain alkane and LCFA, respectively. The results indicated that probe A and probe B had a large stokes shift of 118 nm and 120 nm and high quantum yield of 0.1043 and 0.1058, respectively, which were significantly helpful for the fluorescent labeling. As probe A was more stable than probe B in activated sludge, and the fluorescence intensity keep stable during 24 h, probe A was more suitable for labeling in situ. In addition, through the Image Pro Plus 6 (IPP 6) analysis, a quantitative relationship was established between sludge volume index (SVI) and integral optical density (IOD) of the labeled in activated sludge samples. The relationship between IOD and SVI conforms to Logistic curve (² = 0.94).

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