Designing of New Optical Immunosensors Based on 2-Amino-4-(anthracen-9-yl)-7-hydroxy-4-chromene-3-carbonitrile for the Detection of in the Organs of Fingerlings

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2019 Aug 29

PMID 31459665

Citations 2

Authors

Ellairaja Sundaram

Shenbagavalli Kathiravan

Abhijit Manna

Amutha Chinnaiah

VairathevarSivasamy Vasantha

Affiliations

Soon will be listed here.

Abstract

A one-pot greener methodology has been adopted for the synthesis of a simple 4-chromene core-based fluorescent tag of ()-2-amino-4-(anthracen-9-yl)-7-hydroxy-4-chromene-3-carbonitrile (AHC), and its structure has been analyzed using NMR spectroscopy. The physicochemical properties of AHC were well-studied by UV-vis and fluorescent spectroscopy techniques. As a result of excellent emitting property (ϕ ≈ 0.75), it has been coupled with anti-AH through amide linkage, and the AHC-tagged anti-AH has been used as an immunoassay for the selective detection of in the presence of interfering pathogens. Under optimized conditions, immunosensors could successfully quantify from 4 to 736 CFU/mL, and the LOD was 2 CFU/mL. Saliently, the immunoassay has been successfully demonstrated for the analysis of in the organs of fingerlings, and results have shown a very good agreement with our optimized neat AH fluorimetric titration results.

Citing Articles

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Optical Immunosensor for the Detection of in Food Matrixes.

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