Secondary Structure Determination by Means of ATR-FTIR Spectroscopy

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2017 Jul 30

PMID 28755370

Citations 10

Authors

Batoul Srour

Stefan Bruechert

Susana L A Andrade

Petra Hellwig

Affiliations

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Abstract

Specialized infrared spectroscopic techniques have been developed that allow studying the secondary structure of membrane proteins and the influence of crucial parameters like lipid content and detergent. Here, we focus on an ATR-FTIR spectroscopic study of Af-Amt1 and the influence of LDAO/glycerol on its structural integrity. Our results clearly indicate that infrared spectroscopy can be used to identify the adapted sample conditions.

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