Porcine Familial Adenomatous Polyposis Model Enables Systematic Analysis of Early Events in Adenoma Progression

Overview

Journal Sci Rep

Specialty Science

Date 2017 Jul 28

PMID 28747659

Citations 14

Authors

Tatiana Flisikowska

Monika Stachowiak

Hongen Xu

Alexandra Wagner

Alejandra Hernandez-Caceres

Christine Wurmser

Carolin Perleberg

Hubert Pausch

Anna Perkowska

Konrad Fischer

Dmitrij Frishman

Ruedi Fries

Marek Switonski

Alexander Kind

Dieter Saur

Angelika Schnieke

Krzysztof Flisikowski

Affiliations

Soon will be listed here.

Abstract

We compared gene expression in low and high-grade intraepithelial dysplastic polyps from pigs carrying an APC truncating mutation orthologous to human APC , analysing whole samples and microdissected dysplastic epithelium. Gene set enrichment analysis revealed differential expression of gene sets similar to human normal mucosa versus T1 stage polyps. Transcriptome analysis of whole samples revealed many differentially-expressed genes reflecting immune infiltration. Analysis of microdissected dysplastic epithelium was markedly different and showed increased expression in high-grade intraepithelial neoplasia of several genes known to be involved in human CRC; and revealed possible new roles for GBP6 and PLXND1. The pig model thus facilitates analysis of CRC pathogenesis.

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