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Prokaryotic Argonaute Proteins: Novel Genome-editing Tools?

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Date 2017 Jul 25
PMID 28736447
Citations 79
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Abstract

Argonaute proteins constitute a highly diverse family of nucleic acid-guided proteins. They were first discovered in eukaryotes as key proteins in RNA interference systems, but homologous prokaryotic Argonaute proteins (pAgos) have also been found in archaea and bacteria. In this Progress article, we focus on long pAgo variants, a class of pAgos that are involved in nucleic acid-guided host defence against invading nucleic acids, and discuss the potential of pAgos in genome editing.

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References
1.
Burroughs A, Iyer L, Aravind L . Two novel PIWI families: roles in inter-genomic conflicts in bacteria and Mediator-dependent modulation of transcription in eukaryotes. Biol Direct. 2013; 8:13. PMC: 3702460. DOI: 10.1186/1745-6150-8-13. View

2.
Sheng G, Zhao H, Wang J, Rao Y, Tian W, Swarts D . Structure-based cleavage mechanism of Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage. Proc Natl Acad Sci U S A. 2013; 111(2):652-7. PMC: 3896195. DOI: 10.1073/pnas.1321032111. View

3.
Yuan Y, Pei Y, Ma J, Kuryavyi V, Zhadina M, Meister G . Crystal structure of A. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage. Mol Cell. 2005; 19(3):405-19. PMC: 4689305. DOI: 10.1016/j.molcel.2005.07.011. View

4.
Zander A, Holzmeister P, Klose D, Tinnefeld P, Grohmann D . Single-molecule FRET supports the two-state model of Argonaute action. RNA Biol. 2014; 11(1):45-56. PMC: 3929424. DOI: 10.4161/rna.27446. View

5.
Qi J, Dong Z, Shi Y, Wang X, Qin Y, Wang Y . NgAgo-based fabp11a gene knockdown causes eye developmental defects in zebrafish. Cell Res. 2016; 26(12):1349-1352. PMC: 5143420. DOI: 10.1038/cr.2016.134. View