Identification of QTLs Controlling Grain Protein Concentration Using a High-density SNP and SSR Linkage Map in Barley (Hordeum Vulgare L.)

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2017 Jul 13

PMID 28697758

Citations 9

Authors

Chaofeng Fan

Huijie Zhai

Huifang Wang

Yafei Yue

Minghu Zhang

Jinghui Li

Shaozhe Wen

Ganggang Guo

Yawen Zeng

Zhongfu Ni

Mingshan You

Affiliations

Soon will be listed here.

Abstract

Background: Grain protein concentration (GPC) is a major determinant of quality in barley (Hordeum vulgare L.). Breeding barley cultivars with high GPC has practical value for feed and food properties. The aim of the present study was to identify quantitative trait loci (QTLs) for GPC that could be detected under multiple environments.

Results: A population of 190 recombinant inbred lines (RILs) deriving from a cross between Chinese landrace ZGMLEL with high GPC (> 20%) and Australian cultivar Schooner was used for linkage and QTL analyses. The genetic linkage map spanned 2353.48 cM in length with an average locus interval of 2.33 cM. GPC was evaluated under six environments for the RIL population and the two parental lines. In total, six environmentally stable QTLs for GPC were detected on chromosomes 2H (1), 4H (1), 6H (1), and 7H (3) and the increasing alleles were derived from ZGMLEL. Notably, the three QTLs on chromosome 7H (QGpc.ZiSc-7H.1, QGpc.ZiSc-7H.2, and QGpc.ZiSc-7H.3) that linked in coupling phase were firstly identified. Moreover, the genetic effects of stable QTLs on chromosomes 2H, 6H and 7H were validated using near isogenic lines (NILs).

Conclusions: Collectively, the identified QTLs expanded our knowledge about the genetic basis of GPC in barley and could be selected to develop cultivars with high grain protein concentration.

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