Development of a New Set of Molecular Markers for Examining Glu-A1 Variants in Common Wheat and Ancestral Species

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2017 Jul 7

PMID 28683152

Citations 2

Authors

Zhenying Dong

Yushuang Yang

Kunpu Zhang

Yiwen Li

Junjun Wang

Zhaojun Wang

Xin Liu

Huanju Qin

Daowen Wang

Affiliations

Soon will be listed here.

Abstract

In common wheat (Triticum aestivum L.), allelic variations of Glu-A1 locus have important influences on grain end-use quality. Among the three Glu-A1 alleles, Glu-A1a and -A1b encode the high-molecular-weight glutenin subunits (HMW-GSs) 1Ax1 and 1Ax2*, respectively, whereas Glu-A1c does not specify any subunit. Here, we detected a total of 11 Glu-A1 locus haplotypes (H1 to H11) in three wheat species, by developing and using a new set of DNA markers (Xrj5, Xid3, Xrj6, Xid4 and Xrj7). The main haplotypes found in the diploid wheat T. urartu were H1, H4, H5 and H6, with H1 and H4 expressing both 1Ax and 1Ay subunits. The major haplotypes revealed for tetraploid wheat (T. turgidum) were H1, H8 and H9, with the lines expressing both 1Ax and 1Ay belonging to H1, H4 or H7. Four major haplotypes (H1, H9, H10 and H11) were discovered in common wheat, with Glu-A1a associated with H1 and H8, Glu-A1b with H10 or H11, and Glu-A1c with H9. The Glu-A1 locus haplotypes and the new set of DNA markers have potential to be used for more effectively studying and utilizing the molecular variations of Glu-A1 to improve the end-use quality of common wheat are discussed.

Citing Articles

Genomic and functional genomics analyses of gluten proteins and prospect for simultaneous improvement of end-use and health-related traits in wheat.

Wang D, Li F, Cao S, Zhang K Theor Appl Genet. 2020; 133(5):1521-1539.

PMID: 32020238 PMC: 7214497. DOI: 10.1007/s00122-020-03557-5.

Allelic variation of high molecular weight glutenin subunits of bread wheat in Hebei province of China.

Gao Z, Tian G, Wang Y, Li Y, Cao Q, Han M J Genet. 2018; 97(4):905-910.

PMID: 30262702

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