Comparative Muscle Transcriptome Associated with Carcass Traits of Nellore Cattle

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2017 Jul 5

PMID 28673252

Citations 26

Authors

Barbara Silva-Vignato

Luiz L Coutinho

Aline S M Cesar

Mirele D Poleti

Luciana C A Regitano

Julio C C Balieiro

Affiliations

Soon will be listed here.

Abstract

Background: Commercial cuts yield is an important trait for beef production, which affects the final value of the products, but its direct determination is a challenging procedure to be implemented in practice. The measurement of ribeye area (REA) and backfat thickness (BFT) can be used as indirect measures of meat yield. REA and BFT are important traits studied in beef cattle due to their strong implication in technological (carcass yield) and nutritional characteristics of meat products, like the degree of muscularity and total body fat. Thus, the aim of this work was to study the Longissimus dorsi muscle transcriptome of Nellore cattle, associated with REA and BFT, to find differentially expressed (DE) genes, metabolic pathways, and biological processes that may regulate these traits.

Results: By comparing the gene expression level between groups with extreme genomic estimated breeding values (GEBV), 101 DE genes for REA and 18 for BFT (false discovery rate, FDR 10%) were identified. Functional enrichment analysis for REA identified two KEGG pathways, MAPK (Mitogen-Activated Protein Kinase) signaling pathway and endocytosis pathway, and three biological processes, response to endoplasmic reticulum stress, cellular protein modification process, and macromolecule modification. The MAPK pathway is responsible for fundamental cellular processes, such as growth, differentiation, and hypertrophy. For BFT, 18 biological processes were found to be altered and grouped into 8 clusters of semantically similar terms. The DE genes identified in the biological processes for BFT were ACHE, SRD5A1, RSAD2 and RSPO3. RSAD2 has been previously shown to be associated with lipid droplet content and lipid biosynthesis.

Conclusion: In this study, we identified genes, metabolic pathways, and biological processes, involved in differentiation, proliferation, protein turnover, hypertrophy, as well as adipogenesis and lipid biosynthesis related to REA and BFT. These results enlighten some of the molecular processes involved in muscle and fat deposition, which are economically important carcass traits for beef production.

Citing Articles

Transcriptomic profiling of backfat and muscle in Lijiang pigs with divergent body size across growth stages.

Fang S, Luo S, Jin S, Liu J, Li J, Zhang Y Sci Rep. 2024; 14(1):27677.

PMID: 39532937 PMC: 11557953. DOI: 10.1038/s41598-024-78054-6.

Comparative Transcriptome Analysis of mRNA and miRNA during the Development of Longissimus Dorsi Muscle of Gannan Yak and Tianzhu White Yak.

Niu Y, Guo D, Wei Y, Li J, Bai Y, Liu Z Animals (Basel). 2024; 14(15).

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Genome-Wide Association (GWAS) Applied to Carcass and Meat Traits of Nellore Cattle.

Reis H, Carvalho M, Espigolan R, Poleti M, Ambrizi D, Berton M Metabolites. 2024; 14(1).

PMID: 38276296 PMC: 10818672. DOI: 10.3390/metabo14010006.

Meat Quality and Muscle Tissue Proteome of Crossbred Bulls Finished under Feedlot Using Wet Distiller Grains By-Product.

Baldassini W, Gagaoua M, Santiago B, Rocha L, Torrecilhas J, Torres R Foods. 2023; 11(20).

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Differential Allele-Specific Expression Revealed Functional Variants and Candidate Genes Related to Meat Quality Traits in Muscle.

Bruscadin J, Cardoso T, Diniz W, de Souza M, Afonso J, Vieira D Genes (Basel). 2022; 13(12).

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