Evaluation of Relationship Between Body Mass Index with Gene Expression and Vitamin D Levels of Follicular Fluid in Overweight Patients with Polycystic Ovary Syndrome

Overview

Journal Int J Fertil Steril

Specialty Reproductive Medicine

Date 2017 Jul 4

PMID 28670428

Citations 9

Authors

Esmat Aghadavod

Hakimeh Mollaei

Mohammad Nouri

Hamed Hamishehkar

Affiliations

Soon will be listed here.

Abstract

Background: Polycystic ovary syndrome (PCOS) is the most common endocrine disorder associated with reproductive disorders and metabolic dysfunctions including insulin resistance. The roles of vitamin D in the regulation of metabolic modulations specifically involving insulin and reproduction processing are introduced. In addition, obesity appears to be closely associated with severity of PCOS. The present study is to evaluate the effect of body mass index (BMI) on vitamin D levels in follicular fluid and expression levels in granulos cells.

Materials And Methods: A comparative study was conducted on 80 women with average age of 20-35 years referred for fertilization (IVF). Patients were divided into four groups, and serum levels of testosterone and insulin resistance (IR) were evaluated at the puncture time. Also, vitamin D levels of follicular fluid were evaluated. gene expression was assayed by quantified-polymerase chain reaction (PCR) technique. Correlations were evaluated with calculation of the Spearman coefficient, and also independent relationships were assessed by means of multiple regression analysis.

Results: Vitamin D levels of follicular fluid decreased in PCOS patients compared with non-PCOS. Also, over-weight individuals had lower vitamin D levels compared with normal-weight patients. Vitamin D levels of follicular fluid were highly correlated with BMI (r=-0.51, P<0.01). Homeostatic model assessment-IR (HOMA-IR) values were significantly higher in women of PCOS/overweight and PCOS/normal weight in comparison with women of non-PCOS/normal weight (P<0.01). The gene expression data of in granulosa cells were significantly lower in the PCOS/overweight group compared with the non-PCOS/normal weight (P<0.01).

Conclusion: The findings indicated significant differences in gene expression in granulosa cells and vitamin D of follicular fluid in PCOS/overweight patients.

Citing Articles

Causal association between low vitamin D and polycystic ovary syndrome: a bidirectional mendelian randomization study.

Gao B, Zhang C, Wang D, Li B, Shan Z, Teng W J Ovarian Res. 2024; 17(1):95.

PMID: 38715063 PMC: 11077756. DOI: 10.1186/s13048-024-01420-5.

Vitamin D and polycystic ovary syndrome (PCOS): a review.

Mohan A, Haider R, Fakhor H, Hina F, Kumar V, Jawed A Ann Med Surg (Lond). 2023; 85(7):3506-3511.

PMID: 37427232 PMC: 10328709. DOI: 10.1097/MS9.0000000000000879.

Correlation Between Allergic Rhinitis and Body Mass Index: An Observational Study.

Harugop A, Walia A, Havaldar R, Mudhol R Indian J Otolaryngol Head Neck Surg. 2022; 74(Suppl 2):1033-1036.

PMID: 36452591 PMC: 9702266. DOI: 10.1007/s12070-020-02095-2.

The level of vitamin D in follicular fluid and ovarian reserve in an in vitro fertilization program: A pilot study.

Han J, Kim S, Kim H, Ku S Sci Prog. 2022; 105(2):368504221103782.

PMID: 35619572 PMC: 10364929. DOI: 10.1177/00368504221103782.

Effects of Vitamin D on Fertility, Pregnancy and Polycystic Ovary Syndrome-A Review.

Varbiro S, Takacs I, Tuu L, Nas K, Sziva R, Hetthessy J Nutrients. 2022; 14(8).

PMID: 35458211 PMC: 9029121. DOI: 10.3390/nu14081649.

References

Marcotorchino J, Tourniaire F, Astier J, Karkeni E, Canault M, Amiot M . Vitamin D protects against diet-induced obesity by enhancing fatty acid oxidation. J Nutr Biochem. 2014; 25(10):1077-83. DOI: 10.1016/j.jnutbio.2014.05.010. View

Corduk N, Abban G, Yildirim B, Sarioglu-Buke A . The effect of vitamin D on expression of TGF β1 in ovary. Exp Clin Endocrinol Diabetes. 2012; 120(8):490-3. DOI: 10.1055/s-0032-1314858. View

Khan K . Gene expression in Mammalian cells and its applications. Adv Pharm Bull. 2013; 3(2):257-63. PMC: 3848218. DOI: 10.5681/apb.2013.042. View

Kucur C, Kabil Kucur S, Gozukara I, Seven A, Yuksel K, Keskin N . Extended high frequency audiometry in polycystic ovary syndrome. ScientificWorldJournal. 2013; 2013:482689. PMC: 3806435. DOI: 10.1155/2013/482689. View

Salles J, Chanet A, Giraudet C, Patrac V, Pierre P, Jourdan M . 1,25(OH)2-vitamin D3 enhances the stimulating effect of leucine and insulin on protein synthesis rate through Akt/PKB and mTOR mediated pathways in murine C2C12 skeletal myotubes. Mol Nutr Food Res. 2013; 57(12):2137-46. DOI: 10.1002/mnfr.201300074. View

Burris H, Camargo Jr C . Vitamin D and gestational diabetes mellitus. Curr Diab Rep. 2013; 14(1):451. PMC: 3895371. DOI: 10.1007/s11892-013-0451-3. View

Kim J, Choi Y, Chae S, Hwang K, Yoon S, Kim M . Vitamin D deficiency in women with polycystic ovary syndrome. Clin Exp Reprod Med. 2014; 41(2):80-5. PMC: 4102694. DOI: 10.5653/cerm.2014.41.2.80. View

Bouillon R, Suda T . Vitamin D: calcium and bone homeostasis during evolution. Bonekey Rep. 2014; 3:480. PMC: 3899559. DOI: 10.1038/bonekey.2013.214. View

Blomberg Jensen M, Nielsen J, Jorgensen A, Rajpert-De Meyts E, Kristensen D, Jorgensen N . Vitamin D receptor and vitamin D metabolizing enzymes are expressed in the human male reproductive tract. Hum Reprod. 2010; 25(5):1303-11. DOI: 10.1093/humrep/deq024. View

10.

Hong Y, Chen D, Li Y, Li C, Liu Y, Xie M . Serum sex hormone binding globulin profile and its association with insulin resistance in Chinese peri-menopausal women. Endokrynol Pol. 2013; 64(3):197-201. View

11.

Schuch N, Garcia V, Vivolo S, Araujo Martini L . Relationship between Vitamin D Receptor gene polymorphisms and the components of metabolic syndrome. Nutr J. 2013; 12:96. PMC: 3726454. DOI: 10.1186/1475-2891-12-96. View

12.

Nociti Jr F, Foster B, Tran A, Dunn D, Presland R, Wang L . Vitamin D represses dentin matrix protein 1 in cementoblasts and osteocytes. J Dent Res. 2013; 93(2):148-54. PMC: 6728571. DOI: 10.1177/0022034513516344. View

13.

Farzadi L, Nouri M, Ghojazadeh M, Mohiti M, Aghadavod E . Evaluation of ovarian reserve after laparoscopic surgery in patients with polycystic ovary syndrome. Bioimpacts. 2013; 2(3):167-70. PMC: 3648929. DOI: 10.5681/bi.2012.018. View

14.

Brewer C, Balen A . The adverse effects of obesity on conception and implantation. Reproduction. 2010; 140(3):347-64. DOI: 10.1530/REP-09-0568. View

15.

Alebic M, Bulum T, Stojanovic N, Duvnjak L . Definition of insulin resistance using the homeostasis model assessment (HOMA-IR) in IVF patients diagnosed with polycystic ovary syndrome (PCOS) according to the Rotterdam criteria. Endocrine. 2014; 47(2):625-30. DOI: 10.1007/s12020-014-0182-5. View

16.

Danziger J, Biggs M, Niemi M, Ix J, Kizer J, Djousse L . Circulating 25-hydroxyvitamin D is associated with insulin resistance cross-sectionally but not longitudinally in older adults: The Cardiovascular Health Study. Metabolism. 2013; 62(12):1788-94. PMC: 4159161. DOI: 10.1016/j.metabol.2013.07.008. View

17.

Liu W, Chen Y, Golan M, Annunziata M, Du J, Dougherty U . Intestinal epithelial vitamin D receptor signaling inhibits experimental colitis. J Clin Invest. 2013; 123(9):3983-96. PMC: 3754241. DOI: 10.1172/JCI65842. View

18.

Aziz M, Wissing M, Naver K, Faber J, Skouby S . Polycystic ovary syndrome and low-grade inflammation with special reference to YKL-40. Gynecol Endocrinol. 2014; 30(4):311-5. DOI: 10.3109/09513590.2013.879854. View

19.

Smolikova K, Mlynarcikova A, Scsukova S . Effect of 1α,25-dihydroxyvitamin D3 on progesterone secretion by porcine ovarian granulosa cells. Endocr Regul. 2013; 47(3):123-31. DOI: 10.4149/endo_2013_03_123. View

20.

Lerchbaum E, Obermayer-Pietsch B . Vitamin D and fertility: a systematic review. Eur J Endocrinol. 2012; 166(5):765-78. DOI: 10.1530/EJE-11-0984. View