Betaine is Accumulated Via Transient Choline Dehydrogenase Activation During Mouse Oocyte Meiotic Maturation

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2017 Jul 1

PMID 28663368

Citations 6

Authors

Taylor McClatchie

Megan Meredith

Mariame O Ouedraogo

Sandy Slow

Michael Lever

Mellissa R W Mann

Steven H Zeisel

Jacquetta M Trasler

Jay M Baltz

Affiliations

Soon will be listed here.

Abstract

Betaine (-trimethylglycine) plays key roles in mouse eggs and preimplantation embryos first in a novel mechanism of cell volume regulation and second as a major methyl donor in blastocysts, but its origin is unknown. Here, we determined that endogenous betaine was present at low levels in germinal vesicle (GV) stage mouse oocytes before ovulation and reached high levels in the mature, ovulated egg. However, no betaine transport into oocytes was detected during meiotic maturation. Because betaine can be synthesized in mammalian cells via choline dehydrogenase (CHDH; EC 1.1.99.1), we assessed whether this enzyme was expressed and active. transcripts and CHDH protein were expressed in oocytes. No CHDH enzyme activity was detected in GV oocyte lysate, but CHDH became highly active during oocyte meiotic maturation. It was again inactive after fertilization. We then determined whether oocytes synthesized betaine and whether CHDH was required. Isolated maturing oocytes autonomously synthesized betaine in the presence of choline, whereas this failed to occur in oocytes, directly demonstrating a requirement for CHDH for betaine accumulation in oocytes. Overall, betaine accumulation is a previously unsuspected physiological process during mouse oocyte meiotic maturation whose underlying mechanism is the transient activation of CHDH.

Citing Articles

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