Effects of the Probiotic Bifidobacterium Animalis Subsp. Lactis on the Non-surgical Treatment of Periodontitis. A Histomorphometric, Microtomographic and Immunohistochemical Study in Rats

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2017 Jun 30

PMID 28662142

Citations 25

Authors

Milla S T Ricoldi

Flavia A C Furlaneto

Luiz F F Oliveira

Gustavo C Teixeira

Jessica P Pischiotini

Andre L G Moreira

Edilson Ervolino

Marice N de Oliveira

Cristina S B Bogsan

Sergio L Salvador

Michel R Messora

Affiliations

Soon will be listed here.

Abstract

Lactobacillus probiotics have been investigated in periodontitis. However, the effects of the genus Bifidobacterium on periodontitis are hardly known. This study evaluated the effects of the probiotic (PROB) Bifidobacterium animalis subsp. lactis (B. lactis) HN019 as an adjunct to scaling and root planing (SRP) in rats with experimental periodontitis (EP). At baseline, 32 rats were assigned to 4 groups: C (control), PROB, EP-SRP and EP-SRP-PROB. In groups EP-SRP and EP-SRP-PROB, the mandibular first molars of the animals received a ligature. At day 14, the ligatures were removed and SRP was performed. Animals of groups PROB and EP-SRP-PROB were orally administered with 10 mL/day of 109 colony forming units of B. lactis HN019 for 15 days, starting at day 14. Animals were euthanized at day 29. Histomorphometric, microtomographic and immunohistochemical analyses were performed. Microbiological effects of B. lactis on biofilm were also evaluated. Data were statistically analyzed (ANOVA, Tukey; Kruskal-Wallis, Dunn's; Two-tailed t-test; p<0.05). Group EP-SRP-PROB presented reduced alveolar bone resorption and attachment loss when compared with Group EP-SRP (p<0.05). Group EP-SRP-PROB showed significantly fewer osteoclasts, increased expression of anti-inflammatory cytokines and reduced expression of proinflammatory cytokines compared with Group EP-SRP (p<0.05). B. lactis promoted a higher ratio between aerobic and anaerobic bacteria in biofilm samples (p<0.05). B. lactis HN019 may have a role in the treatment of EP in rats, as an adjunct to SRP.

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References

Dereka X, Markopoulou C, Vrotsos I . Role of growth factors on periodontal repair. Growth Factors. 2007; 24(4):260-7. DOI: 10.1080/08977190601060990. View

Prescott S, Wickens K, Westcott L, Jung W, Currie H, Black P . Supplementation with Lactobacillus rhamnosus or Bifidobacterium lactis probiotics in pregnancy increases cord blood interferon-gamma and breast milk transforming growth factor-beta and immunoglobin A detection. Clin Exp Allergy. 2008; 38(10):1606-14. DOI: 10.1111/j.1365-2222.2008.03061.x. View

Liu C, Zhang Z, Dong K, Guo X . Adhesion and immunomodulatory effects of Bifidobacterium lactis HN019 on intestinal epithelial cells INT-407. World J Gastroenterol. 2010; 16(18):2283-90. PMC: 2868223. DOI: 10.3748/wjg.v16.i18.2283. View

Ince G, Gursoy H, Dirikan Ipci S, Cakar G, Emekli-Alturfan E, Yilmaz S . Clinical and Biochemical Evaluation of Lozenges Containing Lactobacillus reuteri as an Adjunct to Non-Surgical Periodontal Therapy in Chronic Periodontitis. J Periodontol. 2015; 86(6):746-54. DOI: 10.1902/jop.2015.140612. View

Furlaneto F, Nunes N, Oliveira Filho I, Frota N, Yamamoto K, Lisboa M . Effects of locally administered tiludronic acid on experimental periodontitis in rats. J Periodontol. 2014; 85(9):1291-301. DOI: 10.1902/jop.2014.130581. View

Messora M, Oliveira L, Foureaux R, Taba Jr M, Zangeronimo M, Furlaneto F . Probiotic therapy reduces periodontal tissue destruction and improves the intestinal morphology in rats with ligature-induced periodontitis. J Periodontol. 2013; 84(12):1818-26. DOI: 10.1902/jop.2013.120644. View

Toiviainen A, Jalasvuori H, Lahti E, Gursoy U, Salminen S, Fontana M . Impact of orally administered lozenges with Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis BB-12 on the number of salivary mutans streptococci, amount of plaque, gingival inflammation and the oral microbiome in healthy adults. Clin Oral Investig. 2014; 19(1):77-83. PMC: 4286650. DOI: 10.1007/s00784-014-1221-6. View

Shah M, Gujjari S, Chandrasekhar V . Evaluation of the effect of probiotic (inersan®) alone, combination of probiotic with doxycycline and doxycycline alone on aggressive periodontitis - a clinical and microbiological study. J Clin Diagn Res. 2013; 7(3):595-600. PMC: 3616592. DOI: 10.7860/JCDR/2013/5225.2834. View

Moore K, de Waal Malefyt R, Coffman R, OGarra A . Interleukin-10 and the interleukin-10 receptor. Annu Rev Immunol. 2001; 19:683-765. DOI: 10.1146/annurev.immunol.19.1.683. View

10.

Teles R, Gursky L, Faveri M, Rosa E, Teles F, Feres M . Relationships between subgingival microbiota and GCF biomarkers in generalized aggressive periodontitis. J Clin Periodontol. 2010; 37(4):313-23. PMC: 2910519. DOI: 10.1111/j.1600-051X.2010.01534.x. View

11.

Oliveira L, Salvador S, Silva P, Furlaneto F, Figueiredo L, Casarin R . Benefits of Bifidobacterium animalis subsp. lactis Probiotic in Experimental Periodontitis. J Periodontol. 2016; 88(2):197-208. DOI: 10.1902/jop.2016.160217. View

12.

Hoffman F, Heimbach J, Sanders M, Hibberd P . Executive summary: scientific and regulatory challenges of development of probiotics as foods and drugs. Clin Infect Dis. 2008; 46 Suppl 2:S53-7. DOI: 10.1086/523342. View

13.

Tekce M, Ince G, Gursoy H, Dirikan Ipci S, Cakar G, Kadir T . Clinical and microbiological effects of probiotic lozenges in the treatment of chronic periodontitis: a 1-year follow-up study. J Clin Periodontol. 2015; 42(4):363-72. DOI: 10.1111/jcpe.12387. View

14.

Periasamy S, Kolenbrander P . Mutualistic biofilm communities develop with Porphyromonas gingivalis and initial, early, and late colonizers of enamel. J Bacteriol. 2009; 191(22):6804-11. PMC: 2772475. DOI: 10.1128/JB.01006-09. View

15.

Caglar E, Kuscu O, Selvi Kuvvetli S, Cildir S, Sandalli N, Twetman S . Short-term effect of ice-cream containing Bifidobacterium lactis Bb-12 on the number of salivary mutans streptococci and lactobacilli. Acta Odontol Scand. 2008; 66(3):154-8. DOI: 10.1080/00016350802089467. View

16.

Haukioja A, Yli-Knuuttila H, Loimaranta V, Kari K, Ouwehand A, Meurman J . Oral adhesion and survival of probiotic and other lactobacilli and bifidobacteria in vitro. Oral Microbiol Immunol. 2006; 21(5):326-32. DOI: 10.1111/j.1399-302X.2006.00299.x. View

17.

Messora M, Pereira L, Foureaux R, Oliveira L, Sordi C, Alves A . Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats. Arch Oral Biol. 2016; 66:108-19. DOI: 10.1016/j.archoralbio.2016.02.014. View

18.

Gill H, Rutherfurd K, Prasad J, Gopal P . Enhancement of natural and acquired immunity by Lactobacillus rhamnosus (HN001), Lactobacillus acidophilus (HN017) and Bifidobacterium lactis (HN019). Br J Nutr. 2000; 83(2):167-76. DOI: 10.1017/s0007114500000210. View

19.

Maekawa T, Hajishengallis G . Topical treatment with probiotic Lactobacillus brevis CD2 inhibits experimental periodontal inflammation and bone loss. J Periodontal Res. 2014; 49(6):785-91. PMC: 4119090. DOI: 10.1111/jre.12164. View

20.

Vanderpool C, Yan F, Polk D . Mechanisms of probiotic action: Implications for therapeutic applications in inflammatory bowel diseases. Inflamm Bowel Dis. 2008; 14(11):1585-96. DOI: 10.1002/ibd.20525. View