X-ray Crystal Structure of a Reiterative Transcription Complex Reveals an Atypical RNA Extension Pathway

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2017 Jun 28

PMID 28652344

Citations 8

Authors

Katsuhiko S Murakami

Yeonoh Shin

Charles L Turnbough Jr

Vadim Molodtsov

Affiliations

Soon will be listed here.

Abstract

Reiterative transcription is a noncanonical form of RNA synthesis in which a nucleotide specified by a single base in the DNA template is repetitively added to the nascent transcript. Here we determined the crystal structure of an RNA polymerase, the bacterial enzyme from , engaged in reiterative transcription during transcription initiation at a promoter resembling the promoter of The structure reveals that the reiterative transcript detours from the dedicated RNA exit channel and extends toward the main channel of the enzyme, thereby allowing RNA extension without displacement of the promoter recognition σ-factor. Nascent transcripts containing reiteratively added G residues are eventually extended by nonreiterative transcription, revealing an atypical pathway for the formation of a transcription elongation complex.

Citing Articles

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