Video Rate Volumetric Ca Imaging Across Cortex Using Seeded Iterative Demixing (SID) Microscopy

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2017 Jun 27

PMID 28650477

Citations 62

Authors

Tobias Nobauer

Oliver Skocek

Alejandro J Pernia-Andrade

Lukas Weilguny

Francisca Martinez Traub

Maxim I Molodtsov

Alipasha Vaziri

Affiliations

Soon will be listed here.

Abstract

Light-field microscopy (LFM) is a scalable approach for volumetric Ca imaging with high volumetric acquisition rates (up to 100 Hz). Although the technology has enabled whole-brain Ca imaging in semi-transparent specimens, tissue scattering has limited its application in the rodent brain. We introduce seeded iterative demixing (SID), a computational source-extraction technique that extends LFM to the mammalian cortex. SID can capture neuronal dynamics in vivo within a volume of 900 × 900 × 260 μm located as deep as 380 μm in the mouse cortex or hippocampus at a 30-Hz volume rate while discriminating signals from neurons as close as 20 μm apart, at a computational cost three orders of magnitude less than that of frame-by-frame image reconstruction. We expect that the simplicity and scalability of LFM, coupled with the performance of SID, will open up a range of applications including closed-loop experiments.

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