A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing

Overview

Journal ACS Appl Mater Interfaces

Publisher American Chemical Society

Specialties Biomedical Engineering
Biotechnology

Date 2017 Jun 23

PMID 28640580

Citations 30

Authors

Mi Li

Haichang Li

Xiangguang Li

Hua Zhu

Zihui Xu

Lianqing Liu

Jianjie Ma

Mingjun Zhang

Affiliations

Soon will be listed here.

Abstract

Biopolymeric hydrogels have drawn increasing research interest in biomaterials due to their tunable physical and chemical properties for both creating bioactive cellular microenvironment and serving as sustainable therapeutic reagents. Inspired by a naturally occurring hydrogel secreted from the carnivorous Sundew plant for trapping insects, here we have developed a bioinspired hydrogel to deliver mitsugumin 53 (MG53), an important protein in cell membrane repair, for chronic wound healing. Both chemical compositions and micro-/nanomorphological properties inherent from the natural Sundew hydrogel were mimicked using sodium alginate and gum arabic with calcium ion-mediated cross-linking. On the basis of atomic force microscopy (AFM) force measurements, an optimal sticky hydrogel scaffold was obtained through orthogonal experimental design. Imaging and mechanical analysis showed the distinct correlation between structural morphology, adhesion characteristics, and mechanical properties of the Sundew-inspired hydrogel. Combined characterization and biochemistry techniques were utilized to uncover the underlying molecular composition involved in the interactions between hydrogel and protein. In vitro drug release experiments confirmed that the Sundew-inspired hydrogel had a biphasic-kinetics release, which can facilitate both fast delivery of MG53 for improving the reepithelization process of the wounds and sustained release of the protein for treating chronic wounds. In vivo experiments showed that the Sundew-inspired hydrogel encapsulating with rhMG53 could facilitate dermal wound healing in mouse model. Together, these studies confirmed that the Sundew-inspired hydrogel has both tunable micro-/nanostructures and physicochemical properties, which enable it as a delivery vehicle for chronic wounding healing. The research may provide a new way to develop biocompatible and tunable biomaterials for sustainable drug release to meet the needs of biological activities.

Citing Articles

Expanded Perlite-Reinforced Alginate Xerogels: A Chemical Approach to Sustainable Building and Packaging Materials.

Damjanovic R, Vuksanovic M, Petrovic M, Radovanovic Z, Stavric M, Heinemann R Gels. 2024; 10(12).

PMID: 39727541 PMC: 11675961. DOI: 10.3390/gels10120782.

Unlocking the potential of flavonoid-infused drug delivery systems for diabetic wound healing with a mechanistic exploration.

Chowdhury A, Mazumder P Inflammopharmacology. 2024; 32(5):2861-2896.

PMID: 39217278 DOI: 10.1007/s10787-024-01561-5.

Pharmaceutical Applications of Biomass Polymers: Review of Current Research and Perspectives.

Bejenaru C, Radu A, Segneanu A, Bita A, Ciocilteu M, Mogosanu G Polymers (Basel). 2024; 16(9).

PMID: 38732651 PMC: 11085205. DOI: 10.3390/polym16091182.

Identification of 2-d shape parameters for waste brick powders from varying milling methods: A discussion of quantitative image analysis.

Sinkhonde D Heliyon. 2024; 10(4):e26666.

PMID: 38420483 PMC: 10901084. DOI: 10.1016/j.heliyon.2024.e26666.

Advancements in materials, manufacturing, propulsion and localization: propelling soft robotics for medical applications.

Bo Y, Wang H, Niu H, He X, Xue Q, Li Z Front Bioeng Biotechnol. 2024; 11:1327441.

PMID: 38260727 PMC: 10800571. DOI: 10.3389/fbioe.2023.1327441.

References

Hoffman A . Hydrogels for biomedical applications. Adv Drug Deliv Rev. 2002; 54(1):3-12. DOI: 10.1016/s0169-409x(01)00239-3. View

Green J, Elisseeff J . Mimicking biological functionality with polymers for biomedical applications. Nature. 2016; 540(7633):386-394. PMC: 8186828. DOI: 10.1038/nature21005. View

Tibbitt M, Dahlman J, Langer R . Emerging Frontiers in Drug Delivery. J Am Chem Soc. 2016; 138(3):704-17. DOI: 10.1021/jacs.5b09974. View

Pussak D, Ponader D, Mosca S, Pompe T, Hartmann L, Schmidt S . Specific adhesion of carbohydrate hydrogel particles in competition with multivalent inhibitors evaluated by AFM. Langmuir. 2014; 30(21):6142-50. DOI: 10.1021/la5010006. View

Prajapati V, Jani G, Moradiya N, Randeria N . Pharmaceutical applications of various natural gums, mucilages and their modified forms. Carbohydr Polym. 2013; 92(2):1685-99. DOI: 10.1016/j.carbpol.2012.11.021. View

Garcia J, Garcia A . Cellular mechanotransduction: sensing rigidity. Nat Mater. 2014; 13(6):539-40. DOI: 10.1038/nmat3996. View

Zhang M, Liu M, Prest H, Fischer S . Nanoparticles secreted from ivy rootlets for surface climbing. Nano Lett. 2008; 8(5):1277-80. DOI: 10.1021/nl0725704. View

Higuchi A, Ling Q, Chang Y, Hsu S, Umezawa A . Physical cues of biomaterials guide stem cell differentiation fate. Chem Rev. 2013; 113(5):3297-328. DOI: 10.1021/cr300426x. View

Choi Y, Choi J, Jung Y, Cho Y . Human gelatin tissue-adhesive hydrogels prepared by enzyme-mediated biosynthesis of DOPA and Fe ion crosslinking. J Mater Chem B. 2020; 2(2):201-209. DOI: 10.1039/c3tb20696c. View

10.

Koutsopoulos S, Zhang S . Two-layered injectable self-assembling peptide scaffold hydrogels for long-term sustained release of human antibodies. J Control Release. 2012; 160(3):451-8. DOI: 10.1016/j.jconrel.2012.03.014. View

11.

Jia Y, Chen K, Lin P, Lieber G, Nishi M, Yan R . Treatment of acute lung injury by targeting MG53-mediated cell membrane repair. Nat Commun. 2014; 5:4387. PMC: 4109002. DOI: 10.1038/ncomms5387. View

12.

Sun J, Wei D, Zhu Y, Zhong M, Zuo Y, Fan H . A spatial patternable macroporous hydrogel with cell-affinity domains to enhance cell spreading and differentiation. Biomaterials. 2014; 35(17):4759-68. DOI: 10.1016/j.biomaterials.2014.02.041. View

13.

Caliari S, Burdick J . A practical guide to hydrogels for cell culture. Nat Methods. 2016; 13(5):405-14. PMC: 5800304. DOI: 10.1038/nmeth.3839. View

14.

Huang Y, Wang Y, Sun L, Agrawal R, Zhang M . Sundew adhesive: a naturally occurring hydrogel. J R Soc Interface. 2015; 12(107). PMC: 4590509. DOI: 10.1098/rsif.2015.0226. View

15.

Raeburn J, Cardoso A, Adams D . The importance of the self-assembly process to control mechanical properties of low molecular weight hydrogels. Chem Soc Rev. 2013; 42(12):5143-56. DOI: 10.1039/c3cs60030k. View

16.

Vermonden T, Censi R, Hennink W . Hydrogels for protein delivery. Chem Rev. 2012; 112(5):2853-88. DOI: 10.1021/cr200157d. View

17.

Draeger A, Schoenauer R, Atanassoff A, Wolfmeier H, Babiychuk E . Dealing with damage: plasma membrane repair mechanisms. Biochimie. 2014; 107 Pt A:66-72. DOI: 10.1016/j.biochi.2014.08.008. View

18.

Kai D, Prabhakaran M, Stahl B, Eblenkamp M, Wintermantel E, Ramakrishna S . Mechanical properties and in vitro behavior of nanofiber-hydrogel composites for tissue engineering applications. Nanotechnology. 2012; 23(9):095705. DOI: 10.1088/0957-4484/23/9/095705. View

19.

Li J, Mooney D . Designing hydrogels for controlled drug delivery. Nat Rev Mater. 2018; 1(12). PMC: 5898614. DOI: 10.1038/natrevmats.2016.71. View

20.

Geim A, Dubonos S, Grigorieva I, Novoselov K, Zhukov A, Shapoval S . Microfabricated adhesive mimicking gecko foot-hair. Nat Mater. 2003; 2(7):461-3. DOI: 10.1038/nmat917. View