Co-metabolic Formation of Substituted Phenylacetic Acids by Styrene-degrading Bacteria

Overview

Journal Biotechnol Rep (Amst)

Specialty Biotechnology

Date 2017 Jun 20

PMID 28626693

Citations 8

Authors

Michel Oelschlagel

Stefan R Kaschabek

Juliane Zimmerling

Michael Schlomann

Dirk Tischler

Affiliations

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Abstract

Some soil bacteria are able to metabolize styrene via initial side-chain oxygenation. This catabolic route is of potential biotechnological relevance due to the occurrence of phenylacetic acid as a central metabolite. The styrene-degrading strains 1CP, ST, and the novel isolates sp. Kp5.2 and sp. CWB2 were investigated with respect to their applicability to co-metabolically produce substituted phenylacetic acids. Isolates were found to differ significantly in substrate tolerance and biotransformation yields. Especially, ST was identified as a promising candidate for the production of several phenylacetic acids. The biotransformation of 4-chlorostyrene with cells of strain ST was shown to be stable over a period of more than 200 days and yielded about 38 mmol g after nearly 350 days. Moreover, 4-chloro-α-methylstyrene was predominantly converted to the ()-enantiomer of the acid with 40% enantiomeric excess.

Citing Articles

Characterization of the Glutathione -Transferases Involved in Styrene Degradation in Gordonia rubripertincta CWB2.

Lienkamp A, Burnik J, Heine T, Hofmann E, Tischler D Microbiol Spectr. 2021; 9(1):e0047421.

PMID: 34319142 PMC: 8552685. DOI: 10.1128/Spectrum.00474-21.

Biochemical Characterization of Phenylacetaldehyde Dehydrogenases from Styrene-degrading Soil Bacteria.

Zimmerling J, Oelschlagel M, Grossmann C, Voitel M, Schlomann M, Tischler D Appl Biochem Biotechnol. 2020; 193(3):650-667.

PMID: 33106986 PMC: 7910268. DOI: 10.1007/s12010-020-03421-8.

Genome-Wide Analysis Reveals Genetic Potential for Aromatic Compounds Biodegradation of .

Yang F, Feng H, Massey I, Huang F, Guo J, Zhang X Biomed Res Int. 2020; 2020:5849123.

PMID: 32596333 PMC: 7273453. DOI: 10.1155/2020/5849123.

Editorial: , a Source of Biocatalytic Tools.

Tischler D, van Berkel W, Fraaije M Front Microbiol. 2019; 10:800.

PMID: 31040839 PMC: 6477052. DOI: 10.3389/fmicb.2019.00800.

Investigation of the co-metabolic transformation of 4-chlorostyrene into 4-chlorophenylacetic acid in ST.

Stuhr A, Hofmann S, Schlomann M, Oelschlagel M Biotechnol Rep (Amst). 2018; 18:e00248.

PMID: 29892568 PMC: 5993464. DOI: 10.1016/j.btre.2018.e00248.

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