Optofluidic Device for the Quantification of Circulating Tumor Cells in Breast Cancer

Overview

Journal Sci Rep

Specialty Science

Date 2017 Jun 18

PMID 28623262

Citations 9

Authors

Eric Pedrol

Manuel Garcia-Algar

Jaume Massons

Moritz Nazarenus

Luca Guerrini

Javier Martinez

Airan Rodenas

Ana Fernandez-Carrascal

Magdalena Aguilo

Laura G Estevez

Isabel Calvo

Ana Olano-Daza

Eduardo Garcia-Rico

Francesc Diaz

Ramon A Alvarez-Puebla

Affiliations

Soon will be listed here.

Abstract

Metastatic cancer patients require a continuous monitoring during the sequential treatment cycles to carefully evaluate their disease evolution. Repetition of biopsies is very invasive and not always feasible. Herein, we design and demonstrate a 3D-flow focusing microfluidic device, where all optics are integrated into the chip, for the fluorescence quantification of CTCs in real samples. To test the chip performance, two cell membrane targets, the epithelial cell adhesion molecule, EpCAM, and the receptor tyrosine-protein kinase, HER2, are selected. The efficiency of the platform is demonstrated on cell lines and in a variety of healthy donors and metastatic-breast cancer patients.

Citing Articles

Computational and experimental microfluidics: Total analysis system for mixing, sorting, and concentrating particles and cells.

Coral D, Attard M, Pedrol E, Sole R, Diaz F, Aguilo M APL Bioeng. 2024; 8(2):026101.

PMID: 38633837 PMC: 11023705. DOI: 10.1063/5.0158648.

Affinity-Based Microfluidics Combined with Atomic Force Microscopy for Isolation and Nanomechanical Characterization of Circulating Tumor Cells.

Deliorman M, Glia A, Qasaimeh M Methods Mol Biol. 2023; 2679:41-66.

PMID: 37300608 DOI: 10.1007/978-1-0716-3271-0_4.

Recent advances in lab-on-a-chip systems for breast cancer metastasis research.

Firatligil-Yildirir B, Yalcin-Ozuysal O, Nonappa Nanoscale Adv. 2023; 5(9):2375-2393.

PMID: 37143816 PMC: 10153489. DOI: 10.1039/d2na00823h.

[Microfluidic strategies for separation and analysis of circulating exosomes].

Chen W, Gan Z, Qin J Se Pu. 2021; 39(9):968-980.

PMID: 34486836 PMC: 9404160. DOI: 10.3724/SP.J.1123.2021.07005.

Emerging Lab-on-a-Chip Approaches for Liquid Biopsy in Lung Cancer: Status in CTCs and ctDNA Research and Clinical Validation.

Carvalho A, Ferreira G, Seixas D, Guimaraes-Teixeira C, Henrique R, Monteiro F Cancers (Basel). 2021; 13(9).

PMID: 33925308 PMC: 8123575. DOI: 10.3390/cancers13092101.

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