Identification of Two Novel Chicken GPR133 Variants and Their Expression in Different Tissues

Overview

Journal Funct Integr Genomics

Publisher Springer

Specialties Genetics
Molecular Biology

Date 2017 Jun 15

PMID 28612136

Citations 1

Authors

Kai Tian

Qihai Xiao

Xueyou Zhang

Xi Lan

Xiaoling Zhao

Yan Wang

Diyan Li

Huadong Yin

Lin Ye

Qing Zhu

Affiliations

Soon will be listed here.

Abstract

GPR133 (G protein-coupled receptor 133) plays significant roles in various physiological processes. Alternatively splicing (AS) variants of GPR133 in many species have been predicted in multiple databases, but there is no available information about the AS events of chicken GPR133 (cGPR133). In the present study, two chicken GPR133 variants, cGPR133-va and cGPR133-vb, were identified by a combination of reverse transcription PCR (RT-PCR) and rapid amplification of cDNA 5'-ends (5' RACE). Sequence analysis shows that cGPR133-va and cGPR133-vb are resulting from different AS modules and their sequences are predicted to encode two distinct putative proteins, respectively. Quantitative real-time PCR (qRT-PCR) analysis revealed that cGPR133-va and cGPR133-vb are widely expressed in different tissues, while exhibiting specific expression profile. Altogether, our results first demonstrate the existence of novel cGPR133 variants and illustrate its transcriptional diversity and their widespread distribution, which provides a foundation for the further research of GPR133.

Citing Articles

The expanding functional roles and signaling mechanisms of adhesion G protein-coupled receptors.

Morgan R, Anderson G, Arac D, Aust G, Balenga N, Boucard A Ann N Y Acad Sci. 2019; 1456(1):5-25.

PMID: 31168816 PMC: 7891679. DOI: 10.1111/nyas.14094.

References

Kriechbaumer V, Wang P, Hawes C, Abell B . Alternative splicing of the auxin biosynthesis gene YUCCA4 determines its subcellular compartmentation. Plant J. 2012; 70(2):292-302. DOI: 10.1111/j.1365-313X.2011.04866.x. View

Liebscher I, Schon J, Petersen S, Fischer L, Auerbach N, Demberg L . A tethered agonist within the ectodomain activates the adhesion G protein-coupled receptors GPR126 and GPR133. Cell Rep. 2014; 9(6):2018-26. PMC: 4277498. DOI: 10.1016/j.celrep.2014.11.036. View

Modrek B, Lee C . A genomic view of alternative splicing. Nat Genet. 2001; 30(1):13-9. DOI: 10.1038/ng0102-13. View

Kim Y, Moon S, Hwang M, Kim D, Oh J, Kim Y . Gene-based copy number variation study reveals a microdeletion at 12q24 that influences height in the Korean population. Genomics. 2012; 101(2):134-8. DOI: 10.1016/j.ygeno.2012.11.002. View

Runfola V, Sebastian S, Dilworth F, Gabellini D . Rbfox proteins regulate tissue-specific alternative splicing of Mef2D required for muscle differentiation. J Cell Sci. 2015; 128(4):631-7. PMC: 4357529. DOI: 10.1242/jcs.161059. View

Krishnan A, Almen M, Fredriksson R, Schioth H . The origin of GPCRs: identification of mammalian like Rhodopsin, Adhesion, Glutamate and Frizzled GPCRs in fungi. PLoS One. 2012; 7(1):e29817. PMC: 3251606. DOI: 10.1371/journal.pone.0029817. View

James A, Syed N, Brown J, Nimmo H . Thermoplasticity in the plant circadian clock: how plants tell the time-perature. Plant Signal Behav. 2012; 7(10):1219-23. PMC: 3493400. DOI: 10.4161/psb.21491. View

Kelemen O, Convertini P, Zhang Z, Wen Y, Shen M, Falaleeva M . Function of alternative splicing. Gene. 2012; 514(1):1-30. PMC: 5632952. DOI: 10.1016/j.gene.2012.07.083. View

Alptekin B, Langridge P, Budak H . Abiotic stress miRNomes in the Triticeae. Funct Integr Genomics. 2016; 17(2-3):145-170. PMC: 5383695. DOI: 10.1007/s10142-016-0525-9. View

10.

Xiao Q, Liu D, Li L, Wang J, Huang K, Liu H . Five novel variants of GPR103 and their expression in different tissues of goose (Anser cygnoides). Comp Biochem Physiol B Biochem Mol Biol. 2014; 171:18-25. DOI: 10.1016/j.cbpb.2014.03.002. View

11.

Haitina T, Olsson F, Stephansson O, Alsio J, Roman E, Ebendal T . Expression profile of the entire family of Adhesion G protein-coupled receptors in mouse and rat. BMC Neurosci. 2008; 9:43. PMC: 2386866. DOI: 10.1186/1471-2202-9-43. View

12.

Filichkin S, Priest H, Givan S, Shen R, Bryant D, Fox S . Genome-wide mapping of alternative splicing in Arabidopsis thaliana. Genome Res. 2009; 20(1):45-58. PMC: 2798830. DOI: 10.1101/gr.093302.109. View

13.

Civelli O, Reinscheid R, Zhang Y, Wang Z, Fredriksson R, Schioth H . G protein-coupled receptor deorphanizations. Annu Rev Pharmacol Toxicol. 2012; 53:127-46. PMC: 5828024. DOI: 10.1146/annurev-pharmtox-010611-134548. View

14.

Kuhnert F, Mancuso M, Shamloo A, Wang H, Choksi V, Florek M . Essential regulation of CNS angiogenesis by the orphan G protein-coupled receptor GPR124. Science. 2010; 330(6006):985-9. PMC: 3099479. DOI: 10.1126/science.1196554. View

15.

Promel S, Langenhan T, Arac D . Matching structure with function: the GAIN domain of adhesion-GPCR and PKD1-like proteins. Trends Pharmacol Sci. 2013; 34(8):470-8. DOI: 10.1016/j.tips.2013.06.002. View

16.

Tunc-Ozdemir M, Urano D, Jaiswal D, Clouse S, Jones A . Direct Modulation of Heterotrimeric G Protein-coupled Signaling by a Receptor Kinase Complex. J Biol Chem. 2016; 291(27):13918-13925. PMC: 4933153. DOI: 10.1074/jbc.C116.736702. View

17.

Mele M, Ferreira P, Reverter F, DeLuca D, Monlong J, Sammeth M . Human genomics. The human transcriptome across tissues and individuals. Science. 2015; 348(6235):660-5. PMC: 4547472. DOI: 10.1126/science.aaa0355. View

18.

Venkatakrishnan A, Flock T, Prado D, Oates M, Gough J, Babu M . Structured and disordered facets of the GPCR fold. Curr Opin Struct Biol. 2014; 27:129-37. DOI: 10.1016/j.sbi.2014.08.002. View

19.

Li M, Sun X, Hua L, Huang Y, Wang J, Cao X . Molecular characterization, alternative splicing and expression analysis of bovine DBC1. Gene. 2013; 527(2):689-93. DOI: 10.1016/j.gene.2013.05.065. View

20.

Arac D, Boucard A, Bolliger M, Nguyen J, Soltis S, Sudhof T . A novel evolutionarily conserved domain of cell-adhesion GPCRs mediates autoproteolysis. EMBO J. 2012; 31(6):1364-78. PMC: 3321182. DOI: 10.1038/emboj.2012.26. View