Individual Nuclei in Polykaryons Can Control Cyclin Distribution and DNA Synthesis

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1985 May 1

PMID 2861089

Citations 10

Authors

J E Celis

A Celis

Affiliations

Soon will be listed here.

Abstract

Nuclear patterns of cyclin (PCNA) distribution that subdivide S-phase (determined using PCNA autoantibodies specific for this protein) as well as [3H]thymidine incorporation followed by autoradiography have been used to determine the S-phase synchrony of homophasic polykaryons produced by polyethylene glycol (PEG)-induced fusion of populations of mitotic transformed human amnion cells (AMA) exhibiting the following average distribution of phases: prophase, 9%, metaphase, 60% (including early and late prometaphase), anaphase, 3.8%, telophase, 26.2% and interphase, 1%. Both synchronous and asynchronous polykaryons were generated from these fusions; the latter being frequently observed only amongst populations of multinucleated cells having three or more nuclei. These results are taken to imply that individual nuclei in these polykaryons can control cyclin distribution and DNA synthesis in spite of the fact that they share a common cytoplasm.

Citing Articles

Besnoitia besnoiti-driven endothelial host cell cycle alteration.

Velasquez Z, Lopez-Osorio S, Pervizaj-Oruqaj L, Herold S, Hermosilla C, Taubert A Parasitol Res. 2020; 119(8):2563-2577.

PMID: 32548739 PMC: 7366594. DOI: 10.1007/s00436-020-06744-x.

Effect of Transportation on Cultured Limbal Epithelial Sheets for Worldwide Treatment of Limbal Stem Cell Deficiency.

Utheim O, Lyberg T, Eidet J, Raeder S, Sehic A, Roald B Sci Rep. 2018; 8(1):10502.

PMID: 30002380 PMC: 6043629. DOI: 10.1038/s41598-018-28553-0.

IKVAV regulates ERK1/2 and Akt signalling pathways in BMMSC population growth and proliferation.

Li B, Qiu T, Zhang P, Wang X, Yin Y, Li S Cell Prolif. 2014; 47(2):133-45.

PMID: 24617901 PMC: 4232901. DOI: 10.1111/cpr.12094.

Gastric Ulcers in Middle-Aged Rats: The Healing Effect of Essential Oil from Citrus aurantium L. (Rutaceae).

Polo C, Moraes T, Pellizzon C, Marques M, Rocha L, Hiruma-Lima C Evid Based Complement Alternat Med. 2012; 2012:509451.

PMID: 23243451 PMC: 3518767. DOI: 10.1155/2012/509451.

Proliferation-sensitive nuclear phosphoprotein "dividin" is synthesized almost exclusively during S phase of the cell cycle in human AMA cells.

Celis J, Nielsen S Proc Natl Acad Sci U S A. 1986; 83(21):8187-90.

PMID: 3464947 PMC: 386892. DOI: 10.1073/pnas.83.21.8187.

References

Johnson R, Harris H . DNA synthesis and mitosis in fused cells. II. HeLa-chick erythrocyte heterokaryons. J Cell Sci. 1969; 5(3):625-43. DOI: 10.1242/jcs.5.3.625. View

Bravo R . Changes in the nuclear distribution of cyclin (PCNA) but not its synthesis depend on DNA replication. EMBO J. 1985; 4(3):655-61. PMC: 554238. DOI: 10.1002/j.1460-2075.1985.tb03679.x. View

Rao P, Johnson R . Mammalian cell fusion: studies on the regulation of DNA synthesis and mitosis. Nature. 1970; 225(5228):159-64. DOI: 10.1038/225159a0. View

Johnson R, Harris H . DNA synthesis and mitosis in fused cells. I. HeLa homokaryons. J Cell Sci. 1969; 5(3):603-24. DOI: 10.1242/jcs.5.3.603. View

Gallardo H, DE LUSTIG E, SCHAJOWICZ F . Growth and maintenance of human giant-cell bone tumors (osteoclastomas) in continuous culture. Oncology. 1970; 24(2):146-59. DOI: 10.1159/000224514. View

Westerveld A, Freeke M . Cell cycle of multinucleate cells after cell fusion. Exp Cell Res. 1971; 65(1):140-4. DOI: 10.1016/s0014-4827(71)80059-9. View

Sandberg A, Sofuni T, Takagi N, Moore G . Chronology and pattern of human chromosome replication, IV. Autoradiographic studies of binucleate cells. Proc Natl Acad Sci U S A. 1966; 56(1):105-10. PMC: 285682. DOI: 10.1073/pnas.56.1.105. View

Bolund L, RINGERTZ N, Harris H . Changes in the cytochemical properties of erythrocyte nuclei reactivated by cell fusion. J Cell Sci. 1969; 4(1):71-87. DOI: 10.1242/jcs.4.1.71. View

Burns E . Synchronous and asynchronous DNA synthesis in multinucleated Ehrlich ascites tumor cells compared with multinucleated cells cultured from frog lung. Exp Cell Res. 1971; 66(1):152-6. DOI: 10.1016/s0014-4827(71)80023-x. View

10.

Graves J . DNA synthesis in heterokaryons formed by fusion of mammalian cells from different species. Exp Cell Res. 1972; 72(2):393-403. DOI: 10.1016/0014-4827(72)90007-9. View

11.

Sheehy P, Winn R, Clarkson B . Asynchronous DNA synthesis and asynchronous mitosis in multinuclear ovarian cancer cells. Cancer Res. 1974; 34(5):991-6. View

12.

Johnson R, Mullinger A . The induction of DNA synthesis in the chick red cell nucleus in heterokaryons during the first cell cycle after fusion with HeLa cells. J Cell Sci. 1975; 18(3):455-90. DOI: 10.1242/jcs.18.3.455. View

13.

Yanishevsky R, Prescott D . Late S phase cells (Chinese hamster ovary) induce early S phase DNA labeling patterns in G1 phase nuclei. Proc Natl Acad Sci U S A. 1978; 75(7):3307-11. PMC: 392764. DOI: 10.1073/pnas.75.7.3307. View

14.

Miyachi K, Fritzler M, Tan E . Autoantibody to a nuclear antigen in proliferating cells. J Immunol. 1978; 121(6):2228-34. View

15.

Bravo R, Celis J . A search for differential polypeptide synthesis throughout the cell cycle of HeLa cells. J Cell Biol. 1980; 84(3):795-802. PMC: 2110573. DOI: 10.1083/jcb.84.3.795. View

16.

Bravo R, Bellatin J, Celis J . [35S]-methionine labelled polypeptides from HELA cells. Coordinates and percentage of some major polypeptides. Cell Biol Int Rep. 1981; 5(1):93-6. DOI: 10.1016/0309-1651(81)90162-4. View

17.

Bravo R, Celis A, Mosses D, Celis J . Distribution of HeLa cells polypeptides in cytoplasts and karyoplasts. Cell Biol Int Rep. 1981; 5(5):479-89. DOI: 10.1016/0309-1651(81)90175-2. View

18.

Bravo R, Fey S, Bellatin J, Larsen P, Arevalo J, Celis J . Identification of a nuclear and of a cytoplasmic polypeptide whose relative proportions are sensitive to changes in the rate of cell proliferation. Exp Cell Res. 1981; 136(2):311-9. DOI: 10.1016/0014-4827(81)90009-4. View

19.

Takasaki Y, Deng J, Tan E . A nuclear antigen associated with cell proliferation and blast transformation. J Exp Med. 1981; 154(6):1899-909. PMC: 2186555. DOI: 10.1084/jem.154.6.1899. View

20.

Bravo R, Celis J . Up-dated catalogue of HeLa cell proteins: percentages and characteristics of the major cell polypeptides labeled with a mixture of 16 14C-labeled amino acids. Clin Chem. 1982; 28(4 Pt 2):766-81. View