The Dipole Potential Correlates with Lipid Raft Markers in the Plasma Membrane of Living Cells

Overview

Journal J Lipid Res

Publisher Elsevier

Specialty Biochemistry

Date 2017 Jun 14

PMID 28607008

Citations 13

Authors

Tamas Kovacs

Gyula Batta

Florina Zakany

Janos Szollosi

Peter Nagy

Affiliations

Soon will be listed here.

Abstract

The dipole potential generating an electric field much stronger than any other type of membrane potential influences a wide array of phenomena, ranging from passive permeation to voltage-dependent conformational changes of membrane proteins. It is generated by the ordered orientation of lipid carbonyl and membrane-attached water dipole moments. Theoretical considerations and indirect experimental evidence obtained in model membranes suggest that the dipole potential is larger in liquid-ordered domains believed to correspond to lipid rafts in cell membranes. Using three different dipole potential-sensitive fluorophores and four different labeling approaches of raft and nonraft domains, we showed that the dipole potential is indeed stronger in lipid rafts than in the rest of the membrane. The magnitude of this difference is similar to that observed between the dipole potential in control and sphingolipid-enriched cells characteristic of Gaucher's disease. The results established that the heterogeneity of the dipole potential in living cell membranes is correlated with lipid rafts and imply that alterations in the lipid composition of the cell membrane in human diseases can lead to substantial changes in the dipole potential.

Citing Articles

Look Beyond Plasma Membrane Biophysics: Revealing Considerable Variability of the Dipole Potential Between Plasma and Organelle Membranes of Living Cells.

Szabo M, Cs Szabo B, Kurtan K, Varga Z, Panyi G, Nagy P Int J Mol Sci. 2025; 26(3).

PMID: 39940660 PMC: 11816637. DOI: 10.3390/ijms26030889.

Inhibition of the H1 voltage-gated proton channel compromises the viability of human polarized macrophages in a polarization- and ceramide-dependent manner.

Kovacs T, Cs Szabo B, Kothalawala R, Szekelyhidi V, Nagy P, Varga Z Front Immunol. 2025; 15:1487578.

PMID: 39742270 PMC: 11685079. DOI: 10.3389/fimmu.2024.1487578.

The Synergy of Thermal and Non-Thermal Effects in Hyperthermic Oncology.

Minnaar C, Szigeti G, Szasz A Cancers (Basel). 2024; 16(23).

PMID: 39682096 PMC: 11639953. DOI: 10.3390/cancers16233908.

Novel insights into the modulation of the voltage-gated potassium channel K1.3 activation gating by membrane ceramides.

Cs Szabo B, Szabo M, Nagy P, Varga Z, Panyi G, Kovacs T J Lipid Res. 2024; 65(8):100596.

PMID: 39019344 PMC: 11367112. DOI: 10.1016/j.jlr.2024.100596.

Cholesterol Changes Interfacial Water Alignment in Model Cell Membranes.

Orlikowska-Rzeznik H, Versluis J, Bakker H, Piatkowski L J Am Chem Soc. 2024; 146(19):13151-13162.

PMID: 38687869 PMC: 11099968. DOI: 10.1021/jacs.4c00474.

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