Pramel7 Mediates Ground-state Pluripotency Through Proteasomal-epigenetic Combined Pathways

Overview

Journal Nat Cell Biol

Specialty Cell Biology

Date 2017 Jun 13

PMID 28604677

Citations 23

Authors

Urs Graf

Elisa A Casanova

Sarah Wyck

Damian Dalcher

Marco Gatti

Eva Vollenweider

Michal J Okoniewski

Fabienne A Weber

Sameera S Patel

Marc W Schmid

Jiwen Li

Jafar Sharif

Guido A Wanner

Haruhiko Koseki

Jiemin Wong

Pawel Pelczar

Lorenza Penengo

Raffaella Santoro

Paolo Cinelli

Affiliations

Soon will be listed here.

Abstract

Naive pluripotency is established in preimplantation epiblast. Embryonic stem cells (ESCs) represent the immortalization of naive pluripotency. 2i culture has optimized this state, leading to a gene signature and DNA hypomethylation closely comparable to preimplantation epiblast, the developmental ground state. Here we show that Pramel7 (PRAME-like 7), a protein highly expressed in the inner cell mass (ICM) but expressed at low levels in ESCs, targets for proteasomal degradation UHRF1, a key factor for DNA methylation maintenance. Increasing Pramel7 expression in serum-cultured ESCs promotes a preimplantation epiblast-like gene signature, reduces UHRF1 levels and causes global DNA hypomethylation. Pramel7 is required for blastocyst formation and its forced expression locks ESCs in pluripotency. Pramel7/UHRF1 expression is mutually exclusive in ICMs whereas Pramel7-knockout embryos express high levels of UHRF1. Our data reveal an as-yet-unappreciated dynamic nature of DNA methylation through proteasome pathways and offer insights that might help to improve ESC culture to reproduce in vitro the in vivo ground-state pluripotency.

Citing Articles

Nuclear receptor-SINE B1 network modulates expanded pluripotency in blastoids and blastocysts.

Wong K, Zeng Y, Tay E, Teo J, Cipta N, Hamashima K Nat Commun. 2024; 15(1):10011.

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Pramel15 facilitates zygotic nuclear DNMT1 degradation and DNA demethylation.

Tan J, Li Y, Li X, Zhu X, Liu L, Huang H Nat Commun. 2024; 15(1):7310.

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TEAD2 initiates ground-state pluripotency by mediating chromatin looping.

Guo R, Dong X, Chen F, Ji T, He Q, Zhang J EMBO J. 2024; 43(10):1965-1989.

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An RNA-dependent and phase-separated active subnuclear compartment safeguards repressive chromatin domains.

Lerra L, Panatta M, Bar D, Zanini I, Tan J, Pisano A Mol Cell. 2024; 84(9):1667-1683.e10.

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Synergistic enhancement of the mouse Pramex1 and Pramel1 in repressing retinoic acid (RA) signaling during gametogenesis.

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