Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23

Overview

Journal Stem Cell Reports

Publisher Cell Press

Specialty Cell Biology

Date 2017 Jun 6

PMID 28579394

Citations 8

Authors

Amander T Clark

Sofia Gkountela

Di Chen

Wanlu Liu

Enrique Sosa

Meena Sukhwani

Jon D Hennebold

Kyle E Orwig

Affiliations

Soon will be listed here.

Abstract

Primordial germ cells (PGCs) are the earliest embryonic progenitors in the germline. Correct formation of PGCs is critical to reproductive health as an adult. Recent work has shown that primate PGCs can be differentiated from pluripotent stem cells; however, a bioassay that supports their identity as transplantable germ cells has not been reported. Here, we adopted a xenotransplantation assay by transplanting single-cell suspensions of human and nonhuman primate embryonic Macaca mulatta (rhesus macaque) testes containing PGCs into the seminiferous tubules of adult busulfan-treated nude mice. We discovered that both human and nonhuman primate embryonic testis are xenotransplantable, generating colonies while not generating tumors. Taken together, this work provides two critical references (molecular and functional) for defining transplantable primate PGCs. These results provide a blueprint for differentiating pluripotent stem cells to transplantable PGC-like cells in a species that is amenable to transplantation and fertility studies.

Citing Articles

Perspectives: Methods for Evaluating Primate Spermatogonial Stem Cells.

Munyoki S, Orwig K Methods Mol Biol. 2023; 2656:341-364.

PMID: 37249880 DOI: 10.1007/978-1-0716-3139-3_18.

Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression.

Pierson Smela M, Kramme C, Fortuna P, Adams J, Su R, Dong E Elife. 2023; 12.

PMID: 36803359 PMC: 9943069. DOI: 10.7554/eLife.83291.

Production and Analysis of Human Primordial Germ Cell-Like Cells.

Mitsunaga S, Shioda K, Hanna J, Isselbacher K, Shioda T Methods Mol Biol. 2020; 2195:125-145.

PMID: 32852762 PMC: 11804835. DOI: 10.1007/978-1-0716-0860-9_10.

The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline Formation.

Chen D, Liu W, Zimmerman J, Pastor W, Kim R, Hosohama L Cell Rep. 2018; 25(13):3591-3602.e5.

PMID: 30590035 PMC: 6342560. DOI: 10.1016/j.celrep.2018.12.011.

Differentiation of primate primordial germ cell-like cells following transplantation into the adult gonadal niche.

Sosa E, Chen D, Rojas E, Hennebold J, Peters K, Wu Z Nat Commun. 2018; 9(1):5339.

PMID: 30559363 PMC: 6297357. DOI: 10.1038/s41467-018-07740-7.

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