WIPI3 and WIPI4 β-propellers Are Scaffolds for LKB1-AMPK-TSC Signalling Circuits in the Control of Autophagy

Overview

Journal Nat Commun

Specialty Biology

Date 2017 Jun 1

PMID 28561066

Citations 102

Authors

Daniela Bakula

Amelie J Muller

Theresia Zuleger

Zsuzsanna Takacs

Mirita Franz-Wachtel

Ann-Katrin Thost

Daniel Brigger

Mario P Tschan

Tancred Frickey

Horst Robenek

Boris Macek

Tassula Proikas-Cezanne

Affiliations

Soon will be listed here.

Abstract

Autophagy is controlled by AMPK and mTOR, both of which associate with ULK1 and control the production of phosphatidylinositol 3-phosphate (PtdIns3P), a prerequisite for autophagosome formation. Here we report that WIPI3 and WIPI4 scaffold the signal control of autophagy upstream of PtdIns3P production and have a role in the PtdIns3P effector function of WIPI1-WIPI2 at nascent autophagosomes. In response to LKB1-mediated AMPK stimulation, WIPI4-ATG2 is released from a WIPI4-ATG2/AMPK-ULK1 complex and translocates to nascent autophagosomes, controlling their size, to which WIPI3, in complex with FIP200, also contributes. Upstream, WIPI3 associates with AMPK-activated TSC complex at lysosomes, regulating mTOR. Our WIPI interactome analysis reveals the scaffold functions of WIPI proteins interconnecting autophagy signal control and autophagosome formation. Our functional kinase screen uncovers a novel regulatory link between LKB1-mediated AMPK stimulation that produces a direct signal via WIPI4, and we show that the AMPK-related kinases NUAK2 and BRSK2 regulate autophagy through WIPI4.

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