Three New Strains in the Probiotic Toolbox Against Gut Pathogen Serotype Typhimurium

Overview

Journal Food Technol Biotechnol

Specialty Biotechnology

Date 2017 Jun 1

PMID 28559733

Citations 16

Authors

Mia Potocnjak

Petra Pusic

Jadranka Frece

Maja Abram

Tamara Jankovic

Ivana Gobin

Affiliations

Soon will be listed here.

Abstract

The benefits of probiotic bacteria have been widely explored. However, fermented foods and digestive system of humans and animals are an inexhaustible source of new potentially probiotic microorganisms. In this study we present three new strains isolated from different dairy products: cow's cheese, sheep's cheese and whey. In order to determine the antibacterial activity of yet unexplored strains against serotype Typhimurium, competition and co-culture tests were done. Furthermore, adhesion of these strains to Caco-2 cells and their influence on the adhesion of were tested. Results showed the potential probiotic activity of isolated strains. strains survived in the presence of 1% bile salts, they possessed acidification ability, antibacterial activity and significantly attenuated the growth of Typhimurium in brain heart infusion broth. All tested strains were able to adhere to Caco-2 cells and significantly impair the adhesion of Typhimurium. All three strains exhibited significant probiotic potential and anti- activity; therefore, further testing on models should follow.

Citing Articles

Probiotic Characterization of Lactic Acid Bacteria from Donkey Feces in China.

Wu Y, Yue S, Yu J, Bian F, Chen G, Zhang Y Animals (Basel). 2025; 15(2).

PMID: 39858207 PMC: 11758317. DOI: 10.3390/ani15020207.

Plant-based probiotic foods: current state and future trends.

DAlmeida A, Neta A, de Andrade-Lima M, de Albuquerque T Food Sci Biotechnol. 2024; 33(15):3401-3422.

PMID: 39493382 PMC: 11525375. DOI: 10.1007/s10068-024-01674-1.

Susceptibility of Foodborne Pathogens to Milk-Origin Lactic Acid Bacteria Supernatants: A Comprehensive Meta-Regression Study.

Fernandes N, Loforte Y, Cadavez V, Gonzales-Barron U Foods. 2024; 13(16).

PMID: 39200561 PMC: 11353683. DOI: 10.3390/foods13162635.

In vitro analysis of postbiotic antimicrobial activity against Candida Species in a minimal synthetic model simulating the gut mycobiota in obesity.

Garcia-Gamboa R, Perfecto-Avalos Y, Gonzalez-Garcia J, Alvarez-Calderon M, Gutierrez-Vilchis A, Garcia-Gonzalez A Sci Rep. 2024; 14(1):16760.

PMID: 39033245 PMC: 11271299. DOI: 10.1038/s41598-024-66806-3.

Alleviating Pentatrichomonas hominis-induced damage in IPEC-J2 cells: the beneficial influence of porcine-derived lactobacilli.

Zhu Y, Cai H, Yan Z, Shen H, Fang S, Wang D Vet Res Commun. 2024; 48(4):2331-2342.

PMID: 38771449 DOI: 10.1007/s11259-024-10414-z.

References

Walencka E, Rozalska S, Sadowska B, Rozalska B . The influence of Lactobacillus acidophilus-derived surfactants on staphylococcal adhesion and biofilm formation. Folia Microbiol (Praha). 2008; 53(1):61-6. DOI: 10.1007/s12223-008-0009-y. View

Tejero-Sarinena S, Barlow J, Costabile A, Gibson G, Rowland I . In vitro evaluation of the antimicrobial activity of a range of probiotics against pathogens: evidence for the effects of organic acids. Anaerobe. 2012; 18(5):530-8. DOI: 10.1016/j.anaerobe.2012.08.004. View

Arena M, Silvain A, Normanno G, Grieco F, Drider D, Spano G . Use of Lactobacillus plantarum Strains as a Bio-Control Strategy against Food-Borne Pathogenic Microorganisms. Front Microbiol. 2016; 7:464. PMC: 4829616. DOI: 10.3389/fmicb.2016.00464. View

Myllyluoma E, Ahonen A, Korpela R, Vapaatalo H, Kankuri E . Effects of multispecies probiotic combination on helicobacter pylori infection in vitro. Clin Vaccine Immunol. 2008; 15(9):1472-82. PMC: 2546684. DOI: 10.1128/CVI.00080-08. View

Kumar A, Kumar D . Characterization of Lactobacillus isolated from dairy samples for probiotic properties. Anaerobe. 2015; 33:117-23. DOI: 10.1016/j.anaerobe.2015.03.004. View

Dimitrov Z, Gotova I, Chorbadjiyska E . characterization of the adhesive factors of selected probiotics to Caco-2 epithelium cell line. Biotechnol Biotechnol Equip. 2015; 28(6):1079-1083. PMC: 4434056. DOI: 10.1080/13102818.2014.969948. View

Marteau P, de Vrese M, Cellier C, Schrezenmeir J . Protection from gastrointestinal diseases with the use of probiotics. Am J Clin Nutr. 2001; 73(2 Suppl):430S-436S. DOI: 10.1093/ajcn/73.2.430s. View

Kaushik J, Kumar A, Duary R, Mohanty A, Grover S, Batish V . Functional and probiotic attributes of an indigenous isolate of Lactobacillus plantarum. PLoS One. 2009; 4(12):e8099. PMC: 2779496. DOI: 10.1371/journal.pone.0008099. View

Ouwehand A, Vesterlund S . Health aspects of probiotics. IDrugs. 2003; 6(6):573-80. View

10.

Lehto E, Salminen S . Inhibition of Salmonella typhimurium adhesion to Caco-2 cell cultures by Lactobacillus strain GG spent culture supernate: only a pH effect?. FEMS Immunol Med Microbiol. 1997; 18(2):125-32. DOI: 10.1111/j.1574-695X.1997.tb01037.x. View

11.

Greene J, Klaenhammer T . Factors involved in adherence of lactobacilli to human Caco-2 cells. Appl Environ Microbiol. 1994; 60(12):4487-94. PMC: 202009. DOI: 10.1128/aem.60.12.4487-4494.1994. View

12.

Bishop A, House D, Perkins T, Baker S, Kingsley R, Dougan G . Interaction of Salmonella enterica serovar Typhi with cultured epithelial cells: roles of surface structures in adhesion and invasion. Microbiology (Reading). 2008; 154(Pt 7):1914-1926. PMC: 2652038. DOI: 10.1099/mic.0.2008/016998-0. View

13.

Baccigalupi L, Di Donato A, Parlato M, Luongo D, Carbone V, Rossi M . Small surface-associated factors mediate adhesion of a food-isolated strain of Lactobacillus fermentum to Caco-2 cells. Res Microbiol. 2005; 156(7):830-6. DOI: 10.1016/j.resmic.2005.05.001. View

14.

Woo T, Oka K, Takahashi M, Hojo F, Osaki T, Hanawa T . Inhibition of the cytotoxic effect of Clostridium difficile in vitro by Clostridium butyricum MIYAIRI 588 strain. J Med Microbiol. 2011; 60(Pt 11):1617-1625. DOI: 10.1099/jmm.0.033423-0. View

15.

Lim S, Im D . Screening and characterization of probiotic lactic acid bacteria isolated from Korean fermented foods. J Microbiol Biotechnol. 2009; 19(2):178-86. DOI: 10.4014/jmb.0804.269. View

16.

Giannasca K, Giannasca P, Neutra M . Adherence of Salmonella typhimurium to Caco-2 cells: identification of a glycoconjugate receptor. Infect Immun. 1996; 64(1):135-45. PMC: 173738. DOI: 10.1128/iai.64.1.135-145.1996. View

17.

Molin G . Probiotics in foods not containing milk or milk constituents, with special reference to Lactobacillus plantarum 299v. Am J Clin Nutr. 2001; 73(2 Suppl):380S-385S. DOI: 10.1093/ajcn/73.2.380s. View

18.

Henson S . Estimating the incidence of food-borne Salmonella and the effectiveness of alternative control measures using the Delphi method. Int J Food Microbiol. 1997; 35(3):195-204. DOI: 10.1016/s0168-1605(96)01235-4. View

19.

Neal-McKinney J, Lu X, Duong T, Larson C, Call D, Shah D . Production of organic acids by probiotic lactobacilli can be used to reduce pathogen load in poultry. PLoS One. 2012; 7(9):e43928. PMC: 3433458. DOI: 10.1371/journal.pone.0043928. View

20.

Andino A, Hanning I . Salmonella enterica: survival, colonization, and virulence differences among serovars. ScientificWorldJournal. 2015; 2015:520179. PMC: 4310208. DOI: 10.1155/2015/520179. View