Application of Advanced Fluorescence Microscopy to the Structure of Meiotic Chromosomes

Overview

Journal Biophys Rev

Publisher Springer

Specialty Biophysics

Date 2017 May 17

PMID 28510112

Citations 3

Authors

Peter M Carlton

Affiliations

Soon will be listed here.

Abstract

Chromosomes undergoing meiosis are defined by a macromolecular protein assembly called the synaptonemal complex which holds homologs together and carries out important meiotic functions. By retaining the molecular specificity, multiplexing ability, and in situ imaging capabilities of fluorescence microscopy, but with vastly increased resolution, 3D-SIM and other superresolution techniques are poised to make significant discoveries about the structure and function of the synaptonemal complex. This review discusses recent developments in this field and poses questions approachable with current and future technology.

Citing Articles

Super-Resolution Microscopy in Studying the Structure and Function of the Cell Nucleus.

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PMID: 29340216 PMC: 5762827.

Meiotic cohesin subunits RAD21L and REC8 are positioned at distinct regions between lateral elements and transverse filaments in the synaptonemal complex of mouse spermatocytes.

Rong M, Matsuda A, Hiraoka Y, Lee J J Reprod Dev. 2016; 62(6):623-630.

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