Long Non-coding RNA PARTICLE Bridges Histone and DNA Methylation

Overview

Journal Sci Rep

Specialty Science

Date 2017 May 13

PMID 28496150

Citations 31

Authors

Valerie Brid OLeary

Sarah Hain

Doris Maugg

Jan Smida

Omid Azimzadeh

Soile Tapio

Saak Victor Ovsepian

Michael John Atkinson

Affiliations

Soon will be listed here.

Abstract

PARTICLE (Gene PARTICL- 'Promoter of MAT2A-Antisense RadiaTion Induced Circulating LncRNA) expression is transiently elevated following low dose irradiation typically encountered in the workplace and from natural sources. This long non-coding RNA recruits epigenetic silencers for cis-acting repression of its neighbouring Methionine adenosyltransferase 2A gene. It now emerges that PARTICLE operates as a trans-acting mediator of DNA and histone lysine methylation. Chromatin immunoprecipitation sequencing (ChIP-seq) and immunological evidence established elevated PARTICLE expression linked to increased histone 3 lysine 27 trimethylation. Live-imaging of dbroccoli-PARTICLE revealing its dynamic association with DNA methyltransferase 1 was confirmed by flow cytometry, immunoprecipitation and direct competitive binding interaction through electrophoretic mobility shift assay. Acting as a regulatory docking platform, the long non-coding RNA PARTICLE serves to interlink epigenetic modification machineries and represents a compelling innovative component necessary for gene silencing on a global scale.

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