Characterization of Product and Potential Mechanism of Cr(VI) Reduction by Anaerobic Activated Sludge in a Sequencing Batch Reactor

Overview

Journal Sci Rep

Specialty Science

Date 2017 May 12

PMID 28490749

Citations 2

Authors

Ruofei Jin

Yao Liu

Guangfei Liu

Tian Tian

Sen Qiao

Jiti Zhou

Affiliations

Soon will be listed here.

Abstract

Bioremediation of Cr(VI) and nitrate is considered as a promising and cost-effective alternative to chemical and physical methods. However, organo-Cr(III) complexes in effluent generally causes environmental concerns due to second-pollution. Here, Cr(VI) reduction and immobilization efficiencies of anaerobic activated sludge were investigated. Anaerobic activated sludge showed strong reduction ability of Cr(VI) and possessed a great potential of Cr(III) immobilization. Almost 100.0 mg l Cr(VI) could be completely reduced and immobilized by anaerobic activated sludge in a sequencing batch reactor in 24 h. And most generated Cr(III) was accumulated outside of sludge cells. Extracellular polymeric substances (EPS) could bind to Cr(VI) and form EPS-Cr(VI) interaction to reduce the toxic effect of Cr(VI) and promote the Cr(VI) reduction. Protein-like and humic-like substances were responsible for binding with Cr(VI), meanwhile the process was a thermodynamically favorable binding reaction. Then Cr(VI) was reduced to Cr(III) by membrane-associated chromate reductase of sludge. Eventually, the generated Cr(III) might exist as poly-nuclear Cr(III) complexes adhered to sludge surfaces.

Citing Articles

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References

Henriques I, Love N . The role of extracellular polymeric substances in the toxicity response of activated sludge bacteria to chemical toxins. Water Res. 2007; 41(18):4177-85. DOI: 10.1016/j.watres.2007.05.001. View

Dogan N, Kantar C, Gulcan S, Dodge C, Coskun Yilmaz B, Mazmanci M . Chromium(VI) bioremoval by Pseudomonas bacteria: role of microbial exudates for natural attenuation and biotreatment of Cr(VI) contamination. Environ Sci Technol. 2011; 45(6):2278-85. DOI: 10.1021/es102095t. View

More T, Yadav J, Yan S, Tyagi R, Surampalli R . Extracellular polymeric substances of bacteria and their potential environmental applications. J Environ Manage. 2014; 144:1-25. DOI: 10.1016/j.jenvman.2014.05.010. View

Sahinkaya E, KiliC A . Heterotrophic and elemental-sulfur-based autotrophic denitrification processes for simultaneous nitrate and Cr(VI) reduction. Water Res. 2014; 50:278-86. DOI: 10.1016/j.watres.2013.12.005. View

Chen W, Westerhoff P, Leenheer J, Booksh K . Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter. Environ Sci Technol. 2004; 37(24):5701-10. DOI: 10.1021/es034354c. View

Gustafsson J, Persson I, Oromieh A, van Schaik J, Sjostedt C, Berggren Kleja D . Chromium(III) complexation to natural organic matter: mechanisms and modeling. Environ Sci Technol. 2014; 48(3):1753-61. DOI: 10.1021/es404557e. View

Puzon G, Roberts A, Kramer D, Xun L . Formation of soluble organo-chromium(III) complexes after chromate reduction in the presence of cellular organics. Environ Sci Technol. 2005; 39(8):2811-7. DOI: 10.1021/es048967g. View

Chen H, Ahsan S, Santiago-Berrios M, Abruna H, Webb W . Mechanisms of quenching of Alexa fluorophores by natural amino acids. J Am Chem Soc. 2010; 132(21):7244-5. PMC: 2892418. DOI: 10.1021/ja100500k. View

Tian F, Jiang F, Han X, Xiang C, Ge Y, Li J . Synthesis of a novel hydrazone derivative and biophysical studies of its interactions with bovine serum albumin by spectroscopic, electrochemical, and molecular docking methods. J Phys Chem B. 2010; 114(46):14842-53. DOI: 10.1021/jp105766n. View

10.

Novotnik B, Zuliani T, Scancar J, Milacic R . Inhibition of the nitrification process in activated sludge by trivalent and hexavalent chromium, and partitioning of hexavalent chromium between sludge compartments. Chemosphere. 2014; 105:87-94. DOI: 10.1016/j.chemosphere.2013.12.096. View

11.

Li J, Jin R, Liu G, Tian T, Wang J, Zhou J . Simultaneous removal of chromate and nitrate in a packed-bed bioreactor using biodegradable meal box as carbon source and biofilm carriers. Bioresour Technol. 2016; 207:308-14. DOI: 10.1016/j.biortech.2016.02.005. View

12.

Chen Y, Gu G . Short-term batch studies on biological removal of chromium from synthetic wastewater using activated sludge biomass. Bioresour Technol. 2005; 96(15):1722-9. DOI: 10.1016/j.biortech.2004.12.023. View

13.

Sheng G, Yu H, Li X . Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: a review. Biotechnol Adv. 2010; 28(6):882-94. DOI: 10.1016/j.biotechadv.2010.08.001. View

14.

Kantar C, Demiray H, Dogan N . Role of microbial exopolymeric substances (EPS) on chromium sorption and transport in heterogeneous subsurface soils: II. Binding of Cr(III) in EPS/soil system. Chemosphere. 2010; 82(10):1496-505. DOI: 10.1016/j.chemosphere.2010.11.001. View

15.

Lecomte P, Ehrhardt J . Behavior of hexavalent chromium in a polluted groundwater: redox processes and immobilization in soils. Environ Sci Technol. 2001; 35(7):1350-7. DOI: 10.1021/es001073l. View

16.

Sheng G, Xu J, Luo H, Li W, Li W, Yu H . Thermodynamic analysis on the binding of heavy metals onto extracellular polymeric substances (EPS) of activated sludge. Water Res. 2012; 47(2):607-14. DOI: 10.1016/j.watres.2012.10.037. View

17.

Priester J, Olson S, Webb S, Neu M, Hersman L, Holden P . Enhanced exopolymer production and chromium stabilization in Pseudomonas putida unsaturated biofilms. Appl Environ Microbiol. 2006; 72(3):1988-96. PMC: 1393198. DOI: 10.1128/AEM.72.3.1988-1996.2006. View

18.

Aquino S, Stuckey D . Soluble microbial products formation in anaerobic chemostats in the presence of toxic compounds. Water Res. 2003; 38(2):255-66. DOI: 10.1016/j.watres.2003.09.031. View

19.

Cheng L, Li X, Jiang R, Wang C, Yin H . Effects of Cr(VI) on the performance and kinetics of the activated sludge process. Bioresour Technol. 2010; 102(2):797-804. DOI: 10.1016/j.biortech.2010.08.116. View

20.

Sheng G, Yu H, Yue Z . Production of extracellular polymeric substances from Rhodopseudomonas acidophila in the presence of toxic substances. Appl Microbiol Biotechnol. 2005; 69(2):216-22. DOI: 10.1007/s00253-005-1990-6. View