The NAG Sensor NagC Regulates LEE Gene Expression and Contributes to Gut Colonization by O157:H7

Overview

Journal Front Cell Infect Microbiol

Specialties Cell Biology
Infectious Diseases
Microbiology

Date 2017 May 10

PMID 28484684

Citations 15

Authors

Guillaume Le Bihan

Jean-Felix Sicard

Philippe Garneau

Annick Bernalier-Donadille

Alain P Gobert

Annie Garrivier

Christine Martin

Anthony G Hay

Francis Beaudry

Josee Harel

Gregory Jubelin

Affiliations

Soon will be listed here.

Abstract

Enterohemorrhagic (EHEC) O157:H7 are human pathogens responsible for bloody diarrhea and renal failures. EHEC employ a type 3 secretion system to attach directly to the human colonic epithelium. This structure is encoded by the locus of enterocyte effacement (LEE) whose expression is regulated in response to specific nutrients. In this study, we show that the mucin-derived sugars N-acetylglucosamine (NAG) and N-acetylneuraminic acid (NANA) inhibit EHEC adhesion to epithelial cells through down-regulation of LEE expression. The effect of NAG and NANA is dependent on NagC, a transcriptional repressor of the NAG catabolism in . We show that NagC is an activator of the LEE1 operon and a critical regulator for the colonization of mice intestine by EHEC. Finally, we demonstrate that NAG and NANA as well as the metabolic activity of affect the fitness of EHEC in a NagC-dependent manner. This study highlights the role of NagC in coordinating metabolism and LEE expression in EHEC and in promoting EHEC colonization .

Citing Articles

A novel small RNA regulates Locus of Enterocyte Effacement and site-specific colonization of enterohemorrhagic O157:H7 in gut.

Han R, Qian Y, Zheng C Front Cell Infect Microbiol. 2025; 14:1517328.

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Carbapenem-resistant uses mucus metabolism to facilitate gastrointestinal colonization.

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Carbapenem-Resistant Uses Mucus Metabolism to Facilitate Gastrointestinal Colonization.

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Animal and In Vitro Models as Powerful Tools to Decipher the Effects of Enteric Pathogens on the Human Gut Microbiota.

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