Allele Replacement in Escherichia Coli by Use of a Selectable Marker for Resistance to Spectinomycin: Replacement of the LexA Gene

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1988 Dec 1

PMID 2848016

Citations 7

Authors

S A Hill

J W Little

Affiliations

Soon will be listed here.

Abstract

We replaced the Escherichia coli lexA gene by a segment of DNA coding for resistance to spectinomycin and streptomycin. The use of this segment expands the range of selectable markers usable for allele replacement. The availability of this null lexA mutation will facilitate genetic analysis of lexA and the SOS regulon.

Citing Articles

Use of adaptive laboratory evolution to discover key mutations enabling rapid growth of Escherichia coli K-12 MG1655 on glucose minimal medium.

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Phenotypes of lexA mutations in Salmonella enterica: evidence for a lethal lexA null phenotype due to the Fels-2 prophage.

Bunny K, Liu J, Roth J J Bacteriol. 2002; 184(22):6235-49.

PMID: 12399494 PMC: 151935. DOI: 10.1128/JB.184.22.6235-6249.2002.

A phenotype for enigmatic DNA polymerase II: a pivotal role for pol II in replication restart in UV-irradiated Escherichia coli.

Rangarajan S, Woodgate R, Goodman M Proc Natl Acad Sci U S A. 1999; 96(16):9224-9.

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The genetic requirements for UmuDC-mediated cold sensitivity are distinct from those for SOS mutagenesis.

Opperman T, Murli S, Walker G J Bacteriol. 1996; 178(15):4400-11.

PMID: 8755866 PMC: 178205. DOI: 10.1128/jb.178.15.4400-4411.1996.

One-step cloning system for isolation of bacterial lexA-like genes.

Calero S, Garriga X, Barbe J J Bacteriol. 1991; 173(22):7345-50.

PMID: 1938926 PMC: 209243. DOI: 10.1128/jb.173.22.7345-7350.1991.

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