Site-directed Insertion and Deletion Mutagenesis with Cloned Fragments in Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1985 Mar 1

PMID 2982787

Citations 192

Authors

S C Winans

S J Elledge

J H Krueger

G C Walker

Affiliations

Soon will be listed here.

Abstract

A mutation of a cloned gene that has been made by introducing a transposon or some other selectable genetic determinant can be crossed into the gene's original replicon by linearizing the cloned DNA and transforming a recB recC sbcB mutant. A number of applications of this method are described with genes of either chromosomal or plasmid origin.

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