Co-culturing of Fungal Strains Against As a Model for the Induction of Chemical Diversity and Therapeutic Agents

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2017 May 5

PMID 28469610

Citations 17

Authors

Rachel Serrano

Victor Gonzalez-Menendez

Lorena Rodriguez

Jesus Martin

Jose R Tormo

Olga Genilloud

Affiliations

Soon will be listed here.

Abstract

New fungal SMs (SMs) have been successfully described to be produced by means of -simulated microbial community interactions. Co-culturing of fungi has proved to be an efficient way to induce cell-cell interactions that can promote the activation of cryptic pathways, frequently silent when the strains are grown in laboratory conditions. Filamentous fungi represent one of the most diverse microbial groups known to produce bioactive natural products. Triggering the production of novel antifungal compounds in fungi could respond to the current needs to fight health compromising pathogens and provide new therapeutic solutions. In this study, we have selected the fungus as a model to establish microbial interactions with a large set of fungal strains related to ecosystems where they can coexist with this phytopathogen, and to generate a collection of extracts, obtained from their antagonic microbial interactions and potentially containing new bioactive compounds. The antifungal specificity of the extracts containing compounds induced after interaction was determined against two human fungal pathogens ( and ) and three phytopathogens (, , and ). In addition, their cytotoxicity was also evaluated against the human hepatocellular carcinoma cell line (HepG2). We have identified by LC-MS the production of a wide variety of known compounds induced from these fungal interactions, as well as novel molecules that support the potential of this approach to generate new chemical diversity and possible new therapeutic agents.

Citing Articles

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Enhancing dendrobine production in through mono-culturing of endophytic fungi, (MD33) in a temporary immersion bioreactor system.

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In Vitro Analysis of the Antagonistic Biological and Chemical Interactions between the Endophyte and the Phytopathogen .

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OSMAC Approach and Cocultivation for the Induction of Secondary Metabolism of the Fungus .

Rodriguez Martin-Aragon V, Trigal Martinez M, Cuadrado C, Hernandez Daranas A, Fernandez Medarde A, Sanchez Lopez J ACS Omega. 2023; 8(42):39873-39885.

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Induction of New Aromatic Polyketides from the Marine Actinobacterium through an OSMAC Approach.

Rodriguez Martin-Aragon V, Millan F, Cuadrado C, Hernandez Daranas A, Fernandez Medarde A, Sanchez Lopez J Mar Drugs. 2023; 21(10).

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