Development and Characterization of a Porcine Mitral Valve Scaffold for Tissue Engineering

Overview

Journal J Cardiovasc Transl Res

Publisher Springer

Specialty Cardiology & Vascular Diseases

Date 2017 May 3

PMID 28462436

Citations 11

Authors

M Granados

L Morticelli

S Andriopoulou

P Kalozoumis

M Pflaum

P Iablonskii

B Glasmacher

M Harder

J Hegermann

C Wrede

I Tudorache

S Cebotari

A Hilfiker

A Haverich

Sotirios Korossis

Affiliations

Soon will be listed here.

Abstract

Decellularized scaffolds represent a promising alternative for mitral valve (MV) replacement. This work developed and characterized a protocol for the decellularization of whole MVs. Porcine MVs were decellularized with 0.5% (w/v) SDS and 0.5% (w/v) SD and sterilized with 0.1% (v/v) PAA. Decellularized samples were seeded with human foreskin fibroblasts and human adipose-derived stem cells to investigate cellular repopulation and infiltration, and with human colony-forming endothelial cells to investigate collagen IV formation. Histology revealed an acellular scaffold with a generally conserved histoarchitecture, but collagen IV loss. Following decellularization, no significant changes were observed in the hydroxyproline content, but there was a significant reduction in the glycosaminoglycan content. SEM/TEM analysis confirmed cellular removal and loss of some extracellular matrix components. Collagen and elastin were generally preserved. The endothelial cells produced newly formed collagen IV on the non-cytotoxic scaffold. The protocol produced acellular scaffolds with generally preserved histoarchitecture, biochemistry, and biomechanics.

Citing Articles

Structural and biomechanical characterizations of acellular porcine mitral valve scaffolds: anterior leaflets, posterior leaflets, and chordae tendineae.

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Characterization of a Decellularized Sheep Pulmonary Heart Valves and Analysis of Their Capability as a Xenograft Initial Matrix Material in Heart Valve Tissue Engineering.

Inal M, Darcan C, Akpek A Bioengineering (Basel). 2023; 10(8).

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Development of a dual-component infection-resistant arterial replacement for small-caliber reconstructions: A proof-of-concept study.

Zia S, Djalali-Cuevas A, Pflaum M, Hegermann J, Dipresa D, Kalozoumis P Front Bioeng Biotechnol. 2023; 11:957458.

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Effects of Chemical and Radiation Sterilisation on the Biological and Biomechanical Properties of Decellularised Porcine Peripheral Nerves.

Holland J, Webster G, Rooney P, Wilshaw S, Jennings L, Berry H Front Bioeng Biotechnol. 2021; 9:660453.

PMID: 34150728 PMC: 8209421. DOI: 10.3389/fbioe.2021.660453.

Covalent functionalization of decellularized tissues accelerates endothelialization.

Dal Sasso E, Zamuner A, Filippi A, Romanato F, Palmosi T, Vedovelli L Bioact Mater. 2021; 6(11):3851-3864.

PMID: 33937589 PMC: 8065253. DOI: 10.1016/j.bioactmat.2021.04.003.

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