Translocation of Rubella Virus Glycoprotein E1 into the Endoplasmic Reticulum

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1988 Nov 1

PMID 2845137

Citations 16

Authors

T C Hobman

R Shukin

S Gillam

Affiliations

Soon will be listed here.

Abstract

Rubella virus (RV) contains four structural proteins, C (capsid), E2a, E2b, and E1, which are derived from posttranslational processing of a single polyprotein precursor, p110. C protein is nonglycosylated and is thought to interact with RV RNA to form a nucleocapsid. E1 and E2 are membrane glycoproteins that form the spike complexes located on the virion exterior. Two different E1 cDNAs were used to analyze the requirements for translocation of E1 into the endoplasmic reticulum. Analysis of expression of these cDNAs both in vivo and in vitro showed that RV E1 was stably expressed and glycosylated in COS cells and correctly targeted into microsomes in the absence of E2 glycoprotein. The results provide experimental evidence that translocation of RV E1 glycoprotein into the endoplasmic reticulum is mediated by a signal peptide contained within the 69 carboxyl-terminal residues of E2.

Citing Articles

Rubella virus E2 signal peptide is required for perinuclear localization of capsid protein and virus assembly.

Law L, Duncan R, Esmaili A, Nakhasi H, Hobman T J Virol. 2001; 75(4):1978-83.

PMID: 11160697 PMC: 115144. DOI: 10.1128/JVI.75.4.1978-1983.2001.

Rubella virus replication and links to teratogenicity.

Lee J, Bowden D Clin Microbiol Rev. 2000; 13(4):571-87.

PMID: 11023958 PMC: 88950. DOI: 10.1128/CMR.13.4.571.

A single-amino-acid substitution of a tyrosine residue in the rubella virus E1 cytoplasmic domain blocks virus release.

Yao J, Gillam S J Virol. 2000; 74(7):3029-36.

PMID: 10708417 PMC: 111801. DOI: 10.1128/jvi.74.7.3029-3036.2000.

Mutational analysis, using a full-length rubella virus cDNA clone, of rubella virus E1 transmembrane and cytoplasmic domains required for virus release.

Yao J, Gillam S J Virol. 1999; 73(6):4622-30.

PMID: 10233921 PMC: 112503. DOI: 10.1128/JVI.73.6.4622-4630.1999.

Role of rubella virus glycoprotein domains in assembly of virus-like particles.

Garbutt M, Law L, Chan H, Hobman T J Virol. 1999; 73(5):3524-33.

PMID: 10196241 PMC: 104124. DOI: 10.1128/JVI.73.5.3524-3533.1999.

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