Construction of Influenza Haemagglutinin Genes That Code for Intracellular and Secreted Forms of the Protein

Overview

Journal Nature

Specialty Science

Date 1982 Dec 16

PMID 7144911

Citations 91

Authors

M J Gething

J Sambrook

Affiliations

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Abstract

The DNA sequences encoding the amino-terminal signal peptide or the carboxy-terminal hydrophobic anchor have been deleted from a cloned gene coding for the haemagglutinin (HA) of influenza virus. The wild-type gene has previously been shown to be expressed with high efficiency from simian virus 40 (SV40)-HA recombinant vectors into a fully glycosylated protein that is displayed on the infected cell's surface in an antigenically and biologically active form. The anchor-minus HA also is glycosylated but is secreted efficiently into the medium. By contrast, the signal-minus HA is produced only at low levels, is not glycosylated and is located intracellularly.

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