Nucleotide Sequence Analysis of a Gene Cloned from Leptospira Biflexa Serovar Patoc Which Complements an ArgE Defect in Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1988 Oct 1

PMID 2844724

Citations 7

Authors

R L Zuerner

N W Charon

Affiliations

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Abstract

The genus Leptospira, as a member of the order Spirochaetales, forms one of the most ancient evolutionary branches of the eubacteria. These spirochetes are morphologically and physiologically different from most eubacteria, and little is known about Leptospira genetics. In this communication, we report the first nucleotide sequence of a Leptospira gene. A gene which complements an argE mutation in Escherichia coli was isolated from a plasmid-based genomic library composed of Leptospira biflexa serovar patoc DNA. The functional region for the complementing activity was localized by transposon mutagenesis and restriction enzyme mapping and by subcloning. Nucleotide sequence analysis indicated a single open reading frame within the region containing argE complementing activity. The size of the predicted protein, 31,071 daltons, was in excellent agreement with data obtained from coupled transcription-translation reactions primed with cloned L. biflexa DNA. One surprising result was that the predicted amino acid sequence of this protein closely resembles portions of the beta' subunits of RNA polymerases from bacteria and chloroplasts.

Citing Articles

Leptospirosis.

Levett P Clin Microbiol Rev. 2001; 14(2):296-326.

PMID: 11292640 PMC: 88975. DOI: 10.1128/CMR.14.2.296-326.2001.

Comparison of genetic maps for two Leptospira interrogans serovars provides evidence for two chromosomes and intraspecies heterogeneity.

Zuerner R, Herrmann J, Saint Girons I J Bacteriol. 1993; 175(17):5445-51.

PMID: 7690025 PMC: 206600. DOI: 10.1128/jb.175.17.5445-5451.1993.

Cloning of a hemolysin gene from Leptospira interrogans serovar hardjo.

del Real G, Segers R, van der Zeijst B, Gaastra W Infect Immun. 1989; 57(8):2588-90.

PMID: 2744864 PMC: 313494. DOI: 10.1128/iai.57.8.2588-2590.1989.

Identification and nucleotide sequence of the Leptospira biflexa serovar patoc trpE and trpG genes.

Yelton D, Peng S J Bacteriol. 1989; 171(4):2083-9.

PMID: 2703466 PMC: 209860. DOI: 10.1128/jb.171.4.2083-2089.1989.

Nucleotide sequence analysis of the Leptospira biflexa serovar patoc rpsL and rpsG genes.

Zuerner R, Charon N J Bacteriol. 1990; 172(10):6165-8.

PMID: 2211535 PMC: 526950. DOI: 10.1128/jb.172.10.6165-6168.1990.

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