Effects of Canine and Murine Mesenchymal Stromal Cell Transplantation on Peripheral Nerve Regeneration

Overview

Journal Int J Stem Cells

Date 2017 Apr 28

PMID 28446003

Citations 9

Authors

Diego Noe Rodriguez Sanchez

Matheus Bertanha

Thiago Dias Fernandes

Luiz Antonio de Lima Resende

Elenice Deffune

Rogerio Martins Amorim

Affiliations

Soon will be listed here.

Abstract

Background And Objectives: Maintaining a permissive microenvironment is essential for adequate nerve regeneration. Cell-based therapy has the potential based cell replacement and promotion of axonal growth. The adipose tissue derived mesenchymal stromal cells (Ad-MSC) attract interest because neuroregenerative and anti-inflammatory properties. The aim of this study was to evaluate the effects of canine and murine Ad-MSC transplantation on the sciatic nerve regeneration.

Methods: Forty Wistar rats were divided randomly into: control group - CG (n=8); denervated group - DG (n=8); decellularized vein group - VG (n=8); decellularized vein＋canine MSC-cMSC (n=8); descellularized vein＋murine MSC-mMSC (n=8). After 10-mm nerve gap, the tubulation technique was performed with decellularized vein filled with 10 MSC labeled with quantum dots (Qtracker 665). The sciatic nerve functional index (SFI) and electroneuromyography (ENMG) measurements were carried and morphometric and immunohistochemistry analysis of the tissue.

Results: The SFI values were higher in the cMSC and mMSC groups at day 27 (p<0.020) and day 35 (p<0.011). The ENMG analysis also revealed better results in the mMSC group. Density, number, and total area of the fibers were increased in the mMSC and cMSC groups. Brain-derived neurotrophic factor BDNF and S-100 protein positive immunoreactivity showed a higher expression for both in the nerve of the mMSC and cMSC groups. The MSC labeled with quantum dots were detected at day 35, indicating neuronal survival long after the nerve damage.

Conclusions: Murine and canine Ad-MSC associated with decellularized vein scaffold had positive effects on sciatic nerve regeneration in rats.

Citing Articles

Recent perspectives on the synergy of mesenchymal stem cells with micro/nano strategies in peripheral nerve regeneration-a review.

Sharifi M, Kamalabadi-Farahani M, Salehi M, Ebrahimi-Brough S, Alizadeh M Front Bioeng Biotechnol. 2024; 12:1401512.

PMID: 39050683 PMC: 11266111. DOI: 10.3389/fbioe.2024.1401512.

Artificial Nerve Containing Stem Cells, Vascularity and Scaffold; Review of Our Studies.

Kakinoki R, Akagi M Stem Cell Rev Rep. 2022; 19(2):382-391.

PMID: 36333622 PMC: 9902426. DOI: 10.1007/s12015-022-10467-0.

Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Contribution to Angiogenic Processes in Tissue Regeneration.

Krawczenko A, Klimczak A Int J Mol Sci. 2022; 23(5).

PMID: 35269568 PMC: 8910401. DOI: 10.3390/ijms23052425.

Cell therapy in vascularized composite allotransplantation.

Anggelia M, Cheng H, Lai P, Hsieh Y, Lin C, Lin C Biomed J. 2022; 45(3):454-464.

PMID: 35042019 PMC: 9422067. DOI: 10.1016/j.bj.2022.01.005.

3D-printed nerve guidance conduits multi-functionalized with canine multipotent mesenchymal stromal cells promote neuroregeneration after sciatic nerve injury in rats.

Rodriguez-Sanchez D, Pinto G, Cartarozzi L, de Oliveira A, Bovolato A, de Carvalho M Stem Cell Res Ther. 2021; 12(1):303.

PMID: 34051869 PMC: 8164252. DOI: 10.1186/s13287-021-02315-8.

References

Phinney D, Prockop D . Concise review: mesenchymal stem/multipotent stromal cells: the state of transdifferentiation and modes of tissue repair--current views. Stem Cells. 2007; 25(11):2896-902. DOI: 10.1634/stemcells.2007-0637. View

Spees J, Olson S, Whitney M, Prockop D . Mitochondrial transfer between cells can rescue aerobic respiration. Proc Natl Acad Sci U S A. 2006; 103(5):1283-8. PMC: 1345715. DOI: 10.1073/pnas.0510511103. View

Millesi H . Factors affecting the outcome of peripheral nerve surgery. Microsurgery. 2006; 26(4):295-302. DOI: 10.1002/micr.20242. View

Okuda K, Kawase T, Momose M, Murata M, Saito Y, Suzuki H . Platelet-rich plasma contains high levels of platelet-derived growth factor and transforming growth factor-beta and modulates the proliferation of periodontally related cells in vitro. J Periodontol. 2003; 74(6):849-57. DOI: 10.1902/jop.2003.74.6.849. View

Tos P, Ronchi G, Papalia I, Sallen V, Legagneux J, Geuna S . Chapter 4: Methods and protocols in peripheral nerve regeneration experimental research: part I-experimental models. Int Rev Neurobiol. 2009; 87:47-79. DOI: 10.1016/S0074-7742(09)87004-9. View

Keilhoff G, Fansa H . Mesenchymal stem cells for peripheral nerve regeneration--a real hope or just an empty promise?. Exp Neurol. 2011; 232(2):110-3. DOI: 10.1016/j.expneurol.2011.09.007. View

Caseiro A, Pereira T, Ivanova G, Luis A, Mauricio A . Neuromuscular Regeneration: Perspective on the Application of Mesenchymal Stem Cells and Their Secretion Products. Stem Cells Int. 2016; 2016:9756973. PMC: 4736584. DOI: 10.1155/2016/9756973. View

Cartarozzi L, Spejo A, Ferreira Jr R, Barraviera B, Duek E, Carvalho J . Mesenchymal stem cells engrafted in a fibrin scaffold stimulate Schwann cell reactivity and axonal regeneration following sciatic nerve tubulization. Brain Res Bull. 2015; 112:14-24. DOI: 10.1016/j.brainresbull.2015.01.005. View

Wang J, Ding F, Gu Y, Liu J, Gu X . Bone marrow mesenchymal stem cells promote cell proliferation and neurotrophic function of Schwann cells in vitro and in vivo. Brain Res. 2009; 1262:7-15. DOI: 10.1016/j.brainres.2009.01.056. View

10.

Boyd J, Gordon T . A dose-dependent facilitation and inhibition of peripheral nerve regeneration by brain-derived neurotrophic factor. Eur J Neurosci. 2002; 15(4):613-26. DOI: 10.1046/j.1460-9568.2002.01891.x. View

11.

Liu Y, Nie L, Zhao H, Zhang W, Zhang Y, Wang S . Conserved dopamine neurotrophic factor-transduced mesenchymal stem cells promote axon regeneration and functional recovery of injured sciatic nerve. PLoS One. 2014; 9(10):e110993. PMC: 4208796. DOI: 10.1371/journal.pone.0110993. View

12.

Fu S, Gordon T . The cellular and molecular basis of peripheral nerve regeneration. Mol Neurobiol. 1997; 14(1-2):67-116. DOI: 10.1007/BF02740621. View

13.

Hoke A, Gordon T, Zochodne D, Sulaiman O . A decline in glial cell-line-derived neurotrophic factor expression is associated with impaired regeneration after long-term Schwann cell denervation. Exp Neurol. 2002; 173(1):77-85. DOI: 10.1006/exnr.2001.7826. View

14.

Dahlhoff M, Emrich D, Wolf E, Schneider M . Increased activation of the epidermal growth factor receptor in transgenic mice overexpressing epigen causes peripheral neuropathy. Biochim Biophys Acta. 2013; 1832(12):2068-76. DOI: 10.1016/j.bbadis.2013.07.011. View

15.

Polacek M, Bruun J, Elvenes J, Figenschau Y, Martinez I . The secretory profiles of cultured human articular chondrocytes and mesenchymal stem cells: implications for autologous cell transplantation strategies. Cell Transplant. 2010; 20(9):1381-93. DOI: 10.3727/096368910X550215. View

16.

Kingham P, Kolar M, Novikova L, Novikov L, Wiberg M . Stimulating the neurotrophic and angiogenic properties of human adipose-derived stem cells enhances nerve repair. Stem Cells Dev. 2013; 23(7):741-54. DOI: 10.1089/scd.2013.0396. View

17.

Dinh P, Hazel A, Palispis W, Suryadevara S, Gupta R . Functional assessment after sciatic nerve injury in a rat model. Microsurgery. 2009; 29(8):644-9. DOI: 10.1002/micr.20685. View

18.

Klimaschewski L, Hausott B, Angelov D . The pros and cons of growth factors and cytokines in peripheral axon regeneration. Int Rev Neurobiol. 2013; 108:137-71. DOI: 10.1016/B978-0-12-410499-0.00006-X. View

19.

Liu G, Cheng Y, Guo S, Feng Y, Li Q, Jia H . Transplantation of adipose-derived stem cells for peripheral nerve repair. Int J Mol Med. 2011; 28(4):565-72. DOI: 10.3892/ijmm.2011.725. View

20.

Ehlers M . Deconstructing the axon: Wallerian degeneration and the ubiquitin-proteasome system. Trends Neurosci. 2003; 27(1):3-6. DOI: 10.1016/j.tins.2003.10.015. View