MEDIATOR18 and MEDIATOR20 Confer Susceptibility to Fusarium Oxysporum in Arabidopsis Thaliana

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2017 Apr 26

PMID 28441405

Citations 17

Authors

Thorya Fallath

Brendan N Kidd

Jiri Stiller

Celine Davoine

Stefan Bjorklund

John M Manners

Kemal Kazan

Peer M Schenk

Affiliations

Soon will be listed here.

Abstract

The conserved protein complex known as Mediator conveys transcriptional signals by acting as an intermediary between transcription factors and RNA polymerase II. As a result, Mediator subunits play multiple roles in regulating developmental as well as abiotic and biotic stress pathways. In this report we identify the head domain subunits MEDIATOR18 and MEDIATOR20 as important susceptibility factors for Fusarium oxysporum infection in Arabidopsis thaliana. Mutants of MED18 and MED20 display down-regulation of genes associated with jasmonate signaling and biosynthesis while up-regulation of salicylic acid associated pathogenesis related genes and reactive oxygen producing and scavenging genes. We propose that MED18 and MED20 form a sub-domain within Mediator that controls the balance of salicylic acid and jasmonate associated defense pathways.

Citing Articles

Arabidopsis MORC1 and MED9 Interact to Regulate Defense Gene Expression and Plant Fitness.

Nam J, Bhatt P, Bonnard A, Pujara D, Kang H Plant Pathol J. 2024; 40(5):438-450.

PMID: 39397299 PMC: 11471927. DOI: 10.5423/PPJ.OA.07.2024.0107.

Pseudomonas syringae infectivity correlates to altered transcript and metabolite levels of Arabidopsis mediator mutants.

Blomberg J, Tasselius V, Vergara A, Karamat F, Imran Q, Strand A Sci Rep. 2024; 14(1):6771.

PMID: 38514763 PMC: 10958028. DOI: 10.1038/s41598-024-57192-x.

Combined Transcriptomic and Metabolomic Analysis Reveals Insights into Resistance of Arabidopsis Mutant against the Phytopathogenic Fungus .

Kalogeropoulou E, Aliferis K, Tjamos S, Vloutoglou I, Paplomatas E Plants (Basel). 2022; 11(24).

PMID: 36559570 PMC: 9785915. DOI: 10.3390/plants11243457.

Engineering plant immune circuit: walking to the bright future with a novel toolbox.

Vuong U, Iswanto A, Nguyen Q, Kang H, Lee J, Moon J Plant Biotechnol J. 2022; 21(1):17-45.

PMID: 36036862 PMC: 9829404. DOI: 10.1111/pbi.13916.

The Arabidopsis thaliana-Fusarium oxysporum strain 5176 pathosystem: an overview.

Wang L, Calabria J, Chen H, Somssich M J Exp Bot. 2022; 73(18):6052-6067.

PMID: 35709954 PMC: 9578349. DOI: 10.1093/jxb/erac263.

References

Huffaker A, Ryan C . Endogenous peptide defense signals in Arabidopsis differentially amplify signaling for the innate immune response. Proc Natl Acad Sci U S A. 2007; 104(25):10732-6. PMC: 1965581. DOI: 10.1073/pnas.0703343104. View

Trusov Y, Sewelam N, Rookes J, Kunkel M, Nowak E, Schenk P . Heterotrimeric G proteins-mediated resistance to necrotrophic pathogens includes mechanisms independent of salicylic acid-, jasmonic acid/ethylene- and abscisic acid-mediated defense signaling. Plant J. 2008; 58(1):69-81. DOI: 10.1111/j.1365-313X.2008.03755.x. View

Du Z, Zhou X, Ling Y, Zhang Z, Su Z . agriGO: a GO analysis toolkit for the agricultural community. Nucleic Acids Res. 2010; 38(Web Server issue):W64-70. PMC: 2896167. DOI: 10.1093/nar/gkq310. View

Wigge P . FT, a mobile developmental signal in plants. Curr Biol. 2011; 21(9):R374-8. DOI: 10.1016/j.cub.2011.03.038. View

Kidd B, Edgar C, Kumar K, Aitken E, Schenk P, Manners J . The mediator complex subunit PFT1 is a key regulator of jasmonate-dependent defense in Arabidopsis. Plant Cell. 2009; 21(8):2237-52. PMC: 2751954. DOI: 10.1105/tpc.109.066910. View

Manavella P, Hagmann J, Ott F, Laubinger S, Franz M, Macek B . Fast-forward genetics identifies plant CPL phosphatases as regulators of miRNA processing factor HYL1. Cell. 2012; 151(4):859-870. DOI: 10.1016/j.cell.2012.09.039. View

Koiwa H, Barb A, Xiong L, Li F, McCully M, Lee B . C-terminal domain phosphatase-like family members (AtCPLs) differentially regulate Arabidopsis thaliana abiotic stress signaling, growth, and development. Proc Natl Acad Sci U S A. 2002; 99(16):10893-8. PMC: 125069. DOI: 10.1073/pnas.112276199. View

Plaschka C, Lariviere L, Wenzeck L, Seizl M, Hemann M, Tegunov D . Architecture of the RNA polymerase II-Mediator core initiation complex. Nature. 2015; 518(7539):376-80. DOI: 10.1038/nature14229. View

Smakowska E, Kong J, Busch W, Belkhadir Y . Organ-specific regulation of growth-defense tradeoffs by plants. Curr Opin Plant Biol. 2016; 29:129-37. DOI: 10.1016/j.pbi.2015.12.005. View

10.

Torres M, Jones J, Dangl J . Reactive oxygen species signaling in response to pathogens. Plant Physiol. 2006; 141(2):373-8. PMC: 1475467. DOI: 10.1104/pp.106.079467. View

11.

Asturias F, Jiang Y, Myers L, Gustafsson C, Kornberg R . Conserved structures of mediator and RNA polymerase II holoenzyme. Science. 1999; 283(5404):985-7. DOI: 10.1126/science.283.5404.985. View

12.

Ramakers C, Ruijter J, Lekanne Deprez R, Moorman A . Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. Neurosci Lett. 2003; 339(1):62-6. DOI: 10.1016/s0304-3940(02)01423-4. View

13.

Liao C, Lai Z, Lee S, Yun D, Mengiste T . Arabidopsis HOOKLESS1 Regulates Responses to Pathogens and Abscisic Acid through Interaction with MED18 and Acetylation of WRKY33 and ABI5 Chromatin. Plant Cell. 2016; 28(7):1662-81. PMC: 4981130. DOI: 10.1105/tpc.16.00105. View

14.

Havko N, Major I, Jewell J, Attaran E, Browse J, Howe G . Control of Carbon Assimilation and Partitioning by Jasmonate: An Accounting of Growth-Defense Tradeoffs. Plants (Basel). 2016; 5(1). PMC: 4844420. DOI: 10.3390/plants5010007. View

15.

Lariviere L, Plaschka C, Seizl M, Wenzeck L, Kurth F, Cramer P . Structure of the Mediator head module. Nature. 2012; 492(7429):448-51. DOI: 10.1038/nature11670. View

16.

Kidd B, Cahill D, Manners J, Schenk P, Kazan K . Diverse roles of the Mediator complex in plants. Semin Cell Dev Biol. 2011; 22(7):741-8. DOI: 10.1016/j.semcdb.2011.07.012. View

17.

Cevik V, Kidd B, Zhang P, Hill C, Kiddle S, Denby K . MEDIATOR25 acts as an integrative hub for the regulation of jasmonate-responsive gene expression in Arabidopsis. Plant Physiol. 2012; 160(1):541-55. PMC: 3440227. DOI: 10.1104/pp.112.202697. View

18.

Katsir L, Schilmiller A, Staswick P, He S, Howe G . COI1 is a critical component of a receptor for jasmonate and the bacterial virulence factor coronatine. Proc Natl Acad Sci U S A. 2008; 105(19):7100-5. PMC: 2383947. DOI: 10.1073/pnas.0802332105. View

19.

Cole S, Yoon A, Faull K, Diener A . Host perception of jasmonates promotes infection by Fusarium oxysporum formae speciales that produce isoleucine- and leucine-conjugated jasmonates. Mol Plant Pathol. 2014; 15(6):589-600. PMC: 4211617. DOI: 10.1111/mpp.12117. View

20.

Samanta S, Thakur J . Importance of Mediator complex in the regulation and integration of diverse signaling pathways in plants. Front Plant Sci. 2015; 6:757. PMC: 4584954. DOI: 10.3389/fpls.2015.00757. View